原位缺口翻译技术检测兴安升麻总皂甙的抗突变作用  

IN SITU NICK TRANSLATION METHOD REVEALS THE ANTIMUTAGENIC EFFECT OF THE TOTAL SAPONINS OF CIMICIFUGA DAHURICA

作　　者：林新[1] 李文魁[1] 肖培根[1] 李革[2] 蔡有余[2] 

机构地区：[1]中国医学科学院药用植物资源开发研究所,北京100094 [2]中国医学科学院实验动物研究所,北京100021

出　　处：《癌变．畸变．突变》1993年第4期36-38,13,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：本文采用原位缺口翻译技术,液闪计数测定,观察了兴安升麻总皂甙拮抗诱变剂丝裂霉素C(MMC)对人外周血细胞的致突变作用。结果表明:兴安升麻总皂甙具有很强的抗突变作用,同时提示:此技术是1种快速、灵敏、定量检测DNA损伤的方法。The antimutagenic effect of the total saponins of Cimicifuga dahurica on mutagenicity of normal human peripheral blood cells induced by MMC were quantitatively detected by using in situ nick translation method and liquid scintillation counting. The results showed that the total saponins of Cimicifuga dahurica had strong antimutagenic effect and in situ nick translation method provided a rapid and sensitive quantitative assay for detecting DNA damage.

关 键 词：原位缺口翻译 兴安升麻 总皂甙 

分 类 号：R285.1[医药卫生—中药学]