环氧乙烷的遗传毒性研究 Ⅰ对果蝇伴性隐性致死(SLRL)试验  

GENOTOXICITY STUDY OF ETHYLENE OXIDE I. SEX-LINKED RECESSIVE LETHAL TEST IN DROSOPHILA MELANOGASTER

作　　者：李夏[1] 黄品笺 徐薇[1] 张龄[2] 钟坤[2] 陆静芬[2] 

机构地区：[1]中国科学院上海昆虫研究所,上海200025 [2]上海职工医学院生物学教研室

出　　处：《癌变．畸变．突变》1993年第4期6-9,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金

摘　　要：本文以黑腹果蝇(Drosophila melanogaster)为材料,用吸入法检测了环氧乙烷(Ethylene Oxide简称EtO)对果蝇生殖细胞3个发育阶段(成熟精子,精细胞和精母细胞)的伴性隐性致死(SLRL)的诱变性。环氧乙烷以气体吸入的方式给果蝇染毒,剂量为900mg/m^3和1800mg/m^3,染毒时间为2h。结果:900mg/m^3和1800mg/m^3对果蝇SLRL总的诱变率pt％分别为1.89％和2.84％,在2种浓度的3个阶段中,均是成熟精子的诱变率最高;精细胞其次;精母细胞最低。总诱变率与自发突变率相比较,显示环氧乙烷对果蝇生殖细胞具有一定的诱变作用。We studied the frequency of SLRL mutation in male D.melanogaster (Oregon-K) after inhalation of 900mg/m3 and 1, 800mg/m3 EtO. Three different broods were sampled representing mature sperm, spcrmatids, and spermatocytcs. The background SLRL frequency was 0.03%. The results showed that 1.89% and 2.84% SLRL frequencies were detected at 900mg/ m3and l,800mg/m3 EtO exposure, respectively. The higcst frequencies were recovered from mature sperm followed by spermatids and spermatocytes. The study had demonstrated the ability of EtO to produce heritable genetic damage in the germ cells of D.melanogaster.

关 键 词：环氧乙烷 隐性致死 果蝇 遗传毒性 

分 类 号：R994[医药卫生—毒理学]