小鼠淋巴细胞体外抗弓形虫作用的实验研究  

Experimental study on the in vitro resistance to Toxoplasma gondii by murine lymphocyte

作　　者：石琦[1] 郝炳华[1] 周玉玲[1] 石兴民[1] 杨安宁[1] 张瑾[1] 程彦斌[2] 李哲[2] 

机构地区：[1]西安交通大学医学院免疫学教研室,陕西西安710061 [2]西安交通大学医学院寄生虫学教研室,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2003年第5期493-495,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的 :了解小鼠淋巴细胞体外抗御弓形虫速殖子的效果。方法 :采用大鼠脾细胞培养技术 ,以乙酰螺旋霉素作为阳性对照 ,观察淋巴细胞本身以及加入扁桃酸 (MA)后 ,对虫体侵入淋巴细胞及在淋巴细胞内增殖的影响。结果 :与其他体细胞相比较 ,淋巴细胞在受到弓形虫侵害时 ,仍可抑制和杀灭弓形虫。当存在免疫作用的情况下 ,安全剂量的MA抑制弓形虫侵入的作用不显著 ,但抑制细胞内弓形虫增殖的作用显著。结论 :以细胞介导的免疫作用是宿主抗弓形虫感染的重要因素 ,因此 ,应注重提高机体的细胞免疫 ,同时加用药物 。AIM: To study the activities of in vitro resistance to the tachyzoite of Toxoplasma gondii by murine lymphocytes. METHODS: The rat’s splenocyte culture method was used to observe the effects of lymphocytes themselves and lymphocytes together with Mandelic(MA) on the invasion and proliferation of T. gondii in lymphocytes. At the same time acetylspiramycin was used as positive control. RESULTS: As compared with other somatic cells, the lymphocytes invaded by T.gondii still inhibited and killed the toxoplasma organisms in the presence of immunity, the effect safety dose of MA on inhibition of the invasion of T. gondii was not notable while the inhibition of the proliferation of T. gondii in lymphocytes was remarkable. CONCLUSION: Cell mediated immunity(CMI) was an important factor that host resists the T. gondii infection. So, we should pay attention to improving the organism’s CMI and take proper medicine so as to enhance the effect of lymphocyte’s resistance to T. gondii.

关 键 词：淋巴细胞 扁桃酸 弓形虫 细胞免疫 

分 类 号：R382.5[医药卫生—医学寄生虫学]