国产洋红霉素对P_(388)细胞单链DNA断裂及核仁超微结构的影响  

EFFECTS OF DOMESTIC CARMINOMYCIN ON DNA SINGLE-STRAND BREAKS AND ON NEUCLEOLAR ULTRASTRUCTURAL CHANGES IN P_(388) CELLS

作　　者：颜江华[1] 李常春[1] 黄自强[1] 梁平[2] 

机构地区：[1]福建医学院电镜室,厦门大学抗癌研究中心350040 [2]福建医学院药理教研室,福州350004

出　　处：《癌症》1993年第6期506-509,共4页Chinese Journal of Cancer

摘　　要：用碱洗脱技术观察了国产洋红霉素(CMN)引起小鼠淋巴白血病P_(368)细胞单链DNA的断裂作用。在CMN0.25—2.0μg/ml范围内,这种作用是剂量依赖性的。电镜观察研究CMN对P_(388)细胞核仁超微结构的影响。CMN 1.0μg/ml作用2小时,核仁空泡增多、微球体出现;作用4小时;核仁“帽”形成;作用8小时,部分细胞核仁成分碎裂。With alkaline elution assay, effects of domestic carminomycin on DNA single-strand breaks were observed in leukemia P388 cells. The effects were dose-dependent, timedepen dent and irreversible. Under electron microscope, it was obser/ced that carmino mycin caused a nucleolar 'Cap' forming and a nucleolar segregation or fragmentation.

关 键 词：洋红霉素 核仁 超微结构 P388 

分 类 号：R979.1[医药卫生—药品]