代谢综合征PPARγ C-161T基因多态性与颈动脉损害的相关研究  

Peroxisome proliferator-activated receptor γ C-161T polymorphism and carotid artery atherosclerosis in metabolic syndrome

作　　者：闫振成[1] 祝之明[1] 沈成义[1] 赵志刚[1] 倪银星[1] 钟健[1] 聂海[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所高血压内分泌科重庆市高血压研究所第三军医大学代谢病中心,400042

出　　处：《中华医学杂志》2004年第7期543-547,共5页National Medical Journal of China

基　　金：国家自然科学基金资助项目 (3 972 5 0 13 )

摘　　要：目的　明确代谢综合征 (MS)患者过氧化物酶体增殖物激活受体 γ(PPARγ)C 16 1T基因多态性与颈动脉粥样硬化的关系。方法　检测 2 4 8例MS、16 3例高血压病 (EH)、115例 2型糖尿病 (DM )患者和 12 1名正常对照的体重指数 (BMI)、腰围、血脂、空腹血糖 (FBG)、空腹血浆胰岛素(FINS)、尿酸 (UA)、游离脂肪酸 (FFA)和颈动脉超声 ,聚合酶链反应 限制性片断多态性方法测定PPARγC16 1 T基因多态性。结果　MS组腰围和BMI明显高于对照组、EH组和DM组。MS和EH组血压明显高于对照组和DM组。MS组PPARγCC型 (74 6 % )明显高于对照组 (6 1 1% ) ,T等位基因携带者 (CT +TT) (2 5 4 % )明显低于对照组 (38 8% )和DM组 (34 8% )。MS组CC型颈动脉内膜厚度 (IMT)和斑块指数明显高于CT +TT型 (内膜 :0 84mm± 0 3mm比 0 6 6mm± 0 19mm ;斑块指数 :2 19± 1 2 1比 1 6 6± 1 36 ,P <0 0 5 ) ,EH组和DM组CC型斑块指数明显高于CT +TT型(EH :1 5 5± 1 2 3比 1 2 9± 0 92 ;DM :1 5 7± 1 2比 1 18± 0 85 ,P <0 0 5 ) ,MS组CC型斑块指数明显高于EH和DM组 (P <0 0 1) ,MS组CC型内膜较DM和EH组明显增厚 (P <0 0 5或P <0 0 1)。MS组CC型收缩压和脉压明显高于CT +TT型 [收缩压Objective To assess the relationship between PPARγC161-T polymorphism and Carotid Atherosclerosis in metabolic Syndrome(MS). Methods Polymerase chain reaction-restricted fragments length polmorphism was used to study the distribution of the PPARγC161-T polymorphism in 248 metabolism syndrome ,163 essential hypertension(EH) and 115 type 2 diabetes mellitus(DM) patients and 121 normal controls. Fasting insulin(FINS),fasting blood glucose(FBG), uric Acid (UA),plasma lipids and ultrasonography for carotid artery were examined.Results Waistline and BMI were significantly higher in MS compared with those in control ,EH and DM( P <0.01).systolic blood pressure(SBP) , diastolic blood pressure(DBP) and pulse pressure(PP) were markedly higher in MS and EH compared with those in DM and control( P <0.01). The frequencies of the CC,CT and TT were 74.6%,21.8% and 3.6% in MS respectively, The frequencies of the CC was significantly higher in MS compared with that in control, but T allele carrier (CT+TT) was significantly lower compared with control,DM and EH ( P <0.05 or P <0.01) .In MS,CC genotype had significantly increased the intima-media thickness (IMT) of common carotid artery and plaque index compared with CT+TT (IMT:0.84 mm±0.3 mm vs 0.66 mm±0.19 mm;plaque index:2.19±1.21 vs 1.66±1.36, P <0.05),CC genotype had significantly increased plaque index compared with CT+TT in EH and DM(plaque index:EH:1.55±1.23 vs 1.29±0.92;DM:1.57±1.2 vs 1.18±0.85, P <0.05);CC genotype had significantly higher SBP compared with CT+TT in EH( P <0.05),CC genotype had significantly increased plaque index in MS than that in DM and EH( P <0.01) ,CC genotype had significantly increased IMT in MS compared with DM.CC genotype had significantly higher SBP and PP compared with CT+TT in MS(SBP:155 mm Hg±23 mm Hg vs 145 mm Hg±21 mm Hg;PP:69 mm Hg±8 mm Hg vs 58 mm Hg±8 mm Hg, 1 mm Hg=0.133 kPa, P <0.05)。Conclusion In MS, CC genotype was prone to lesion of carotid artery, but CT+TT may reduce lesion of carotid artery ,which implicates that

关 键 词：代谢综合征 PPARΓ C-161T基因 基因多态性 颈动脉硬化 

分 类 号：R589[医药卫生—内分泌] R543.4[医药卫生—内科学]