成纤维细胞玻璃化保存的初步实验研究  

PRIMARY TESTING STUDY OF PRESERVATION OF FIBROBLAST BY VITRIFICATION

作　　者：程启康[1] 王欣[1] 杨鹏飞[1] 华泽钊[1] 邓辰亮[2] 杨光辉[2] 崔磊[2] 刘伟[2] 曹谊林[2] 

机构地区：[1]上海理工大学低温生物医学与技术研究所,上海200093 [2]上海市组织工程研究与开发中心,上海200235

出　　处：《低温工程》2004年第2期23-26,44,共5页Cryogenics

基　　金：国家自然科学基金重点项目 (5 9836 2 4 0 ) ;973项目 (G19990 5 4 30 9) ;86 3项目 (2 0 0 2AA2 0 5 0 4 1) ;上海市重大科技攻关项目 (0 0DJ14 0 0 1- 6 ) ;上海市教委重点学科建设项目

摘　　要：成纤维细胞是构建组织工程化真皮的种子细胞 ,实现成纤维细胞的低温保存对成功保存组织工程化真皮有着重要的意义。采用不同组成及浓度的玻璃化溶液对成纤维细胞进行玻璃化低温保存实验 ,并考虑了离心次数对细胞存活率的影响。实验结果表明 :玻璃化溶液的组成、浓度及复温后的离心次数等因素对细胞的存活率均有明显的影响。在实验条件下 ,采用组分配比为 5 4mol/LEG+1 4mol/LDMSO的玻璃化溶液对成纤维细胞进行玻璃化保存 ,复温后以 10 0 0r/min的转速离心 1次(5min)后 ,再以 80 0r/min的转速离心 4次 (每次 4min) ,可获得较高的细胞存活率 ,其值为87 1%。Fibroblast is a crucial kind of cell in the construction of tissue engineered dermal equivalent. The cryopreservation of fibroblast has great significance to the tissue-engineered dermis.Different composition and density vitrification solution is used cryopreservate the fibroblast by vitrification.The effects of compositions and concentrations of vitrification solutions, and centrifugation times on the cell viability were examined. The results indicated that these factors that the composition of vitrification solution,density and the centriffugation times after the original temperature had remarkable effects on the cell viability. The survival of cryopreserved fibroblast reaches 87.1% when the vitrification solution contains 5.4mol/L ethylene glycol and 1.4 mol/L DMSO,and the samples were centrifugated at 1 000 r/min for 5 min and at 800 r/min for 4 min (repeat 4 times) after thawing.

关 键 词：成纤维细胞 组织工程化真皮 玻璃化保存 低温保存技术 

分 类 号：Q2[生物学—细胞生物学]