重组人组织型纤溶酶原激活剂改构体复性研究  

Research on reteplase renaturation in vitro

作　　者：冯长根[1] 陈嫚[1] 任启生[2] 宋新荣[2] 

机构地区：[1]北京理工大学新医药开发研究中心,北京100081 [2]北京江中泽生科技有限责任公司,北京100050

出　　处：《中国药学杂志》2004年第5期385-388,共4页Chinese Pharmaceutical Journal

摘　　要：目的 研究重组人组织型纤溶酶原激活剂改构体(reteplase)体外复性。方法 通过研究复性所需的时间、温度、pH值、蛋白浓度、氧化还原对比例和添加精氨酸等因素,确定了reteplase复性所需的基本条件,并对直接稀释法、氧化复性后再稀释和脉冲稀释法进行了方法学比较研究。结果 脉冲稀释法优于直接稀释法和氧化复性后再稀释。结论建立了reteplase复性方法,此方法可使重组蛋白在较高的重折叠浓度下仍具有良好的复性效率。OBJECTIVE: To study on the renaturation of reteplase in vitro. METHODS: The basic renaturation conditions of reteplase were established by analysis of time, temperature, pH, protein concentration, the ratio of reduced and oxidized thiol reagents, adding L-arginine, et al. The traditional dilution, oxidation renaturation before dilution and step-wise dilution were investigated. RESULTS: Compared with the traditional dilution and oxidation renaturation before dilution, the activity of reteplase obtained by step-wise dilution was significantly improved. CONCLUSION: The method of reteplase renaturation was established. The process above can be used to obtain reteplase with high concentration and high activity from Escherichia coli.

关 键 词：重组人组织型纤溶酶原激活剂 改构体 复性 直接稀释 脉冲稀释 

分 类 号：R973.2[医药卫生—药品]