Influence of serum collected from rat perfused with compound Biejiaruangan drug on hepatic stellate cells  

作　　者：Shun-GenGuo WeiZhang TaoJiang MinDai Lu-FenZhang Yi-ChunMeng Li-YunZhao Jian-ZhaoNiu 

机构地区：[1]LaboratoryofCellandBiochemistry,BeijingUniversityofTraditionalChineseMedicine,Beijing100029,China [2]DepartmentofHistologyandEmbryology,MedicalCollegeofChinesePeople'sArmedPoliceForces,Tianjin300162,China [3]CollegeofAcupunctureandMoxibustion,BeijingUniversityofTraditionalChineseMedicine,Beijing100029,China [4]CentralExperimentalLaboratory,BeijingMilitaryMedicineCollege,Beijing100071,China

出　　处：《World Journal of Gastroenterology》2004年第10期1487-1494,共8页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China for Key Project,No.30130220

摘　　要：AIM: To observe the effect of compound Biejiaruangan decoction (CBJRGC) (composite prescription of Carapax trionycis for softening the liver) on proliferation, activation,excretion of collagen and cytokine of hepatic stellate cells (HSCs) and to find the mechanism of prevention and treatment of hepatic fibrosis by CBJRGC.METHODS: Using MTT, immunohistochemistry and image analysis technology, the related indexes for proliferation,activation, excretion of collagen and cytokine of hepatic stellate cells were detected in 24 h, 48 h, and 72 h after adminstration of different dosages of CBJRGC.RESULTS: Statistical analysis showed that serum collected from rat perfused with CBJRGC could restrain the proliferation of HSC in 48 h and 72 h especially in high and medium dosage groups, markedly decrease the expression of desmin, synapsin and platelet derived growth factor (PDGF) in HSC in 24 h, 48 h and 72 h, as well as the expression of μ-SMA, collagen III, TIMP and TGFβ1 in 48 h and 72 h, decrease the excretion of collagen I in 72 h.CBJRGC serum had no significant effect on collagens I, III and TIMP in 24 h.CONCLUSION: CBJRGC serum has a good curative effect on hepatic fibrosis. Its main mechanism may be related to the following factors. The drug serum can restrain the proliferation and activation of HSC, decrease the number of activated HSC and the total number of HSC, the excretion of collagens I, III, enhance the degradation of collagen and restore the balance of synthesis and degradation of collagen,inhibit the expression of transforming growth factor β1 (TGFβI) and platelet derived growth factor (PDGF) in HSC,block and delay the process of hepatic fibrosis. Synapsin is a new marker of activation of HSC, which provides a theoretical and testing basis for neural regulation in the developing process of hepatic fibrosis.AIM: To observe the effect of compound Biejiaruangan decoction (CBJRGC) (composite prescription of Carapax trionycis for softening the liver) on proliferation,activation, excretion of collagen and cytokine of hepatic stellate cells (HSCs) and to find the mechanism of prevention and treatment of hepatic fibrosis by CBJRGC. METHODS: Using MTI,immunohistochemistry and image analysis technology,the related indexes for proliferation, activation,excretion of collagen and cytokine of hepatic stellate cells were detected in 24 h,48 h,and 72 h after adminstration of different dosages of CBJRGC. RESULTS: Statistical analysis showed that serum collected from rat perfused with CBJRGC could restrain the proliferation of HSC in 48 h and 72 h especially in high and medium dosage groups,markedly decrease the expression of desmin,synapsin and platelet derived growth factor (PDGF) in HSC in 24 h,48 h and 72 h,as well as the expression of α-SMA,collagen Ⅲ,TIMP and TGFβ1 in 48 h and 72 h,decrease the excretion of collagen Ⅰ in 72 h. CBJRGC serum had no significant effect on collagens Ⅰ,Ⅲ and TIMP in 24 h. CONCLUSION: CBJRGC serum has a good curative effect on hepatic fibrosis.Its main mechanism may be related to the following factors.The drug serum can restrain the proliferation and activation of HSC,decrease the number of activated HSC and the total number of HSC,the excretion of collagens Ⅰ,Ⅲ,enhance the degradation of collagen and restore the balance of synthesis and degradation of collagen, inhibit the expression of transforming growth factor β1 (TGFβ1) and platelet derived growth factor (PDGF) in HSC, block and delay the process of hepatic fibrosis.Synapsin is a new marker of activation of HSC,which provides a theoretical and testing basis for neural regulation in the developing process of hepatic fibrosis.

关 键 词：肝脏星形细胞 缺血再灌注 血清学检查 肝纤维化 中医药疗法 

分 类 号：R259.752[医药卫生—中西医结合]