人胚神经干细胞的分离培养和鉴定  

Isolation culture and identification of human neural stem cells from human embryos

作　　者：罗树伟[1] 谢常青[1] 卢光琇[1] 

机构地区：[1]中南大学湘雅医学院人类生殖工程研究室

出　　处：《中南大学学报（医学版）》2004年第2期129-131,共3页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金重点项目 (30 0 30 0 70 ) ;国家自然科学基金 (30 1 0 4 0 30 4 8) ;湖南省自然科学基金(OOJJY2 0 36) ;湖南省科技攻关项目 (0 1 99Y1 0 0 1 )

摘　　要：目的 :从流产人胚前脑中分离培养人胚神经干细胞。方法 :神经干细胞具有自我复制和多向分化潜能。本实验利用神经干细胞特定的培养增殖培养体系分离纯化人胚神经干细胞 ,并对分离得到的神经干细胞的分子标记及分化潜能进行鉴定。结果 :从 8～ 1 0周龄流产人胚胎分离得到了神经干细胞 ,它能表达神经干细胞特异性抗原nestin ,并能在体外诱导分化为神经元细胞和神经胶质细胞。结论 :在人胚中枢神经系统中存在神经干细胞 ,并能够在体外扩增 。Objective To obtain and culture the human neural stem cells from the aborted human embryos.Methods The neural stem cells were isolated and purified by the specific proliferous culture system of neural stem cells, and the molecular maker and multi potency of the obtained neural stem cells were identified.Results Human neural stem cells, which were isolated from 8～10 month old aborted human embryos, could express nestin ( a kind of specified antigen of the neural stem cell), and it could be differentiate into neurons and glials.Conclusion Neural stem cells can exist in human embryos, and it can be expanded in vitro.

关 键 词：神经干细胞 细胞培养 细胞分离 细胞鉴定 流产人胚 神经退行性疾病 神经损伤性疾病 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]