皮肤低温储存后纤维连接蛋白和层黏连蛋白表达的研究  

The immunohistochemical study of fibronectin and laminin after cryopreserved human skins

作　　者：贾晓明[1] 王翠娥[2] 吴小红[2] 李豫川[2] 曹军田[2] 甘永华[2] 蔡宏[1] 

机构地区：[1]解放军304医院全军烧伤研究所,北京100037 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《军医进修学院学报》2004年第2期128-130,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金资助项目(30070298)

摘　　要：目的:观察细胞外基质中纤维连接蛋白(FN)、层黏连蛋白(LN)在不同温度储存皮肤组织中的分布,以探讨其与皮肤冷冻损伤的关系。方法:用FN、LN抗体对5种储存条件下的皮肤作免疫组化染色,用图像分析仪测定表皮组织中的含量,并用H.E染色观察皮肤的组织结构变化。结果:与新鲜皮片组相比,-196℃H抗冻液中储存皮肤组织结构保存较好,FN、LN含量变化不明显,而4℃、-20℃、-80℃储存皮肤组织结构破坏明显,含量亦明显下降。结论:皮肤低温储存后冷冻损伤与细胞外基质中FN、LN破坏有关。Objective: To find out the distribution of Fibronectin and Laminin in cryopreserved skins and investigate the relationship with cryoinjury. Methods: Immunohistochemical staining with antibodies of Fibronectin (FN) and Laminin (LN) and Histopathological observation studied 5 groups of skins. Results: Comparing to the normal human skin, the histologieal structure and FN,LN level of skins stored at - 196t in H anti-frozen liquid did not change significantly, while those in groups kept in 4℃ ,-20℃ , -80℃ were destroyed. Conclusion; The skin cryoinjury is correlated with extracellular matrix.

关 键 词：皮肤 低温储存 纤维连接蛋白 层黏连蛋白 细胞外基质 皮肤冷冻损伤 

分 类 号：R645[医药卫生—外科学]