APA微囊化BCCs蛛网膜下腔移植对大鼠慢性神经性疼痛的镇痛效应  

The analgesic effect of APA microcapsulized BCCs subarachnoid transplantation on animal models of neuropathic pain

作　　者：徐龙河[1] 薛毅珑[2] 罗芸[2] 郭水龙[2] 张宏[1] 

机构地区：[1]解放军总医院麻醉科,北京100853 [2]解放军总医院老年医学研究所细胞生物学研究室,北京1000853

出　　处：《军医进修学院学报》2004年第2期131-133,共3页Academic Journal of Pla Postgraduate Medical School

基　　金："十五"军队科研基金项目(01Z031)

摘　　要：目的:观察APA微囊化牛肾上腺嗜铬细胞(BCCs)移植对大鼠慢性神经性疼痛的作用。方法:SD大鼠4组,即对照组(C组),正常大鼠;单纯CCI组,右侧坐骨神经松结扎;CCI注入APA组和APA-BCCs组,CCI模型后7 d,蛛网膜下腔分别移植500～600个APA空囊和5×106个APA微囊化BCCs。测定各组移植后7 d至42 d的触诱发痛阈值(g)和CO2激光刺激痛阈值(ms)。并取脊髓行TH、ENK、DβH免疫组化检测。结果:APA-BCCs组结扎侧触诱发痛阈值和CO2痛阈值在移植后7-42 d均高于CCI组和APA组(P<0.01),与C组无显著差异(P>0.05)。移植后的BCCs TH染色阳性,脊髓软膜可见到DβH和ENK免疫组化染色阳性。结论:APA微囊化BCCs蛛网膜下腔移植可缓解CCI模型大鼠的痛觉过敏和触诱发痛,ENK和NE参与其镇痛作用机制。Objective:To observe the analgesic effect of subarachnoid transplantation of APA microcapsulized BCCs on chronic constriction injury (CCI)rats. Methods; SD rats were randomly divided into four groups ,each has ten. Normal rats were used as control group ( group C). Rats that right sciatic nerve was ligated were used as CGI group . Five to six hundred empty APA microcapsules( group APA) or 5 X 106 APA microcapsulized BCCs ( group APA-BCCs) were grated into subarachnoid space of CCI rats 7 days after operation. Allodynia and hyperalgesia were measured by Von- Frey filaments and CO2 laser dividually one to six weeks after transplantation. TH immunohistochemistry was used to judge the survial of grafted cells. ENK and DpH immuno-histochemistry were done on the spinal cord. Rusults; Allodynia threshold and CO2 pain threshold on the ligated sides of group APA-BCCs were higher than that of on group CCI and APA 7 to 42 days after transplantation, but there were no difference compared with that of group C. Immunohistochemistry showed that TH was positive on grafted BCCs , ENK and DpH were positive on many cells of the pial surface . Conclusion; Subarachnoid transplantation of APA microcapsulized BCCs can alleviate allodynia and heperalgesia in CCI rats. ENK and NE take part in this analgessiic effect.

关 键 词：APA微囊化 BCCs蛛网膜下腔移植 大鼠 慢性神经性疼痛 镇痛效应 牛肾上腺嗜铬细胞 

分 类 号：R441.1[医药卫生—诊断学]