成年大鼠海马p-CREB的分布  

Distribution of p-CREB in hippocampal formation of adult rats

作　　者：于玮[1] 张莉[1] 韩太真[1] 蒋马莉[1] 

机构地区：[1]西安交通大学医学院生理学教研室,陕西西安710061

出　　处：《第四军医大学学报》2004年第10期874-876,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 (30 1 70 31 0 ) ;西安交通大学重点培植基金资助项目 (1 60 ;0 82 0 0 3)

摘　　要：目的 :观察磷酸化CREB(p CREB)在正常成年大鼠海马结构的表达 .方法 :在多聚甲醛固定的海马脑薄片上 ,用免疫组化SP法和免疫荧光法观察 p CREB在正常成年大鼠海马结构的表达与分布 .结果 :p CREB在正常大鼠海马结构有广泛表达 ,在齿状回表达最强 ,CA3区次之 ,CA1区最弱 .阳性表达主要位于胞核 .结论 :p CREB在海马结构齿状回和CA1区的表达是不同的 .由于CREB参与某些形式的长期记忆 ,本结果提示齿状回和海马CA1区可能在长期记忆中的作用不同 .AIM: To investigate the expression and distribution of p CREB in the hippocampal formation of adult rats. METHODS: SP immunohistochemical staining and immunofluorescence were performed in paraformaldehyde fixed hippocampal sections. RESULTS: p CREB was expressed throughout the hippocampal formation, with the expression in the dentate gyrus being the highest, CA3 area, the second, and CA1 area, the least. P CREB was also found to have expressed mostly in nucleus. CONCLUSION: The expressions of p CREB are different in the dentate gyrus and CA1 area, suggesting that dentate gyrus and CA1 area may play different roles in long-term memory.

关 键 词：CREB 海马 免疫组织化学 免疫荧光法 

分 类 号：R338[医药卫生—人体生理学]