鸡血藤煎剂对小鼠骨髓细胞增殖的影响  

Effect of Spatholobus suberectus on the bone marrow cells and related cytokines of mice

作　　者：陈东辉[1] 罗霞[1] 余梦瑶[1] 赵弋清[1] 程耀锋[1] 杨志荣[1] 

机构地区：[1]四川大学生命科学学院

出　　处：《中国中药杂志》2004年第4期352-355,共4页China Journal of Chinese Materia Medica

基　　金：四川省科技厅资助项目 ( 0 3jy0 2 9 10 6)

摘　　要：目的 :探讨鸡血藤调控小鼠骨髓细胞增殖及其“补血”作用的生物学机理。方法 :采用正常小鼠和贫血小鼠模型 ,以造血生长因子生物活性检测技术测定鸡血藤对骨髓细胞增殖的影响。结果 :鸡血藤对正常小鼠和贫血小鼠骨髓细胞增殖有显著促进作用 ;经体内鸡血藤诱导制备的巨噬细胞、脾细胞、肺和骨骼肌体外条件培养液能明显促进正常小鼠和贫血小鼠骨髓细胞增殖。结论 :鸡血藤促进造血的机理可能与直接或间接刺激造血微环境的基质细胞分泌较高活性的造血生长因子有关。Objective:To study the effect of Spatholobus suberectus on proliferation and the hematonic mechanism. Method:The techniques of culture of hematopoietic cell and hematopoietic growth factor(HGF) assay were used. Result: Spatholobus suberectus could obviously promote the proliferation of bone marrow cells in healthy and anaemic mice.The culture media of spleen cell, macrophage, lung and skeletal muscle treated with S. suberectus had much stronger stimulating effects on hematopoietic cells. Conclusion: S. suberectus may enhance hematopoiesis by directly or indirectly stimulating stroma cell in hematopoietic inductive microenvironment and muscle tissue to secrete some HGF(Epo, GM CSF,IL, and MK CSF).This is one of the biological mechanisms for hematonic effect of S. suberectus

关 键 词：鸡血藤煎剂 小鼠 骨髓细胞 细胞增殖 造血生长因子 生物活性检测技术 

分 类 号：R285.5[医药卫生—中药学]