知母皂苷对体外培养成年大鼠海马神经前体细胞增殖的影响  

Effect of saponins from anemarrhena asphodeloides bunge on the cell proliferation in cultured adult hippocampus neural precursor cells in vitro

作　　者：王卫东[1] 程祖珏[1] 邵卫中[1] 康野[1] 徐江平[2] 

机构地区：[1]武警江西总队医院,南昌330001 [2]第一军医大学药理学教研室,广州510514

出　　处：《江西医药》2004年第2期96-98,共3页Jiangxi Medical Journal

摘　　要：目的　观察知母皂苷 (SAaB)对体外培养成年大鼠海马神经前体细胞增殖的影响。方法　体外传代培养的成年大鼠海马神经前体细胞经碱性成纤维细胞生长因子 (bFGF)、知母皂苷处理后 ,采用MTT法测定细胞增殖 ,流式细胞仪测定细胞周期。结果　与对照组相比 ,知母皂苷可明显促进成年大鼠海马神经前体细胞增殖 (P <0 .0 1 ) ,使细胞从G0 /G1 期进入S和G2 /M期 ,其作用效果呈浓度依赖性。结论　知母皂苷可促进成年大鼠海马神经前体细胞增殖 ,其机制可能与知母皂苷的多种生物学活性有关。Objective To observed the effect of saponins from anemarrhena asphodeloides bunge (SAaB) on the cell proliferation in cultured adult hippocampus neural precursor cells in vitro. Methods The passage cultured adult hippocampus neural precursor cells from adult rat in vitro were pretreated by basic fibroblast growth factor (bFGF) and SAaB. Cell proliferation were assessed by MTT colorimetric assay, and cell cycle were analysed by Flow Cytometry. Results Compared with control group, SAaB could significantly increased the cell proliferation actived by bFGF(P<0.01), and the effect depended on the concentration of SAaB. Conclusion The SAaB can promote the proliferation in cultured adult hippocampus neural precursor cells in vitro, and the accommodational mechanisms of enhanced cell proliferation are possibly related to many biological activity of SAaB.

关 键 词：知母皂苷 体外培养 成年大鼠 海马 神经前体细胞 细胞增殖 碱性成纤维细胞生长因子 

分 类 号：R285.5[医药卫生—中药学]