骨疏灵胶囊中异补骨脂素的含量测定  

Content Determination of Isopsoralen in Gushuling Capsule

作　　者：王涛[1] 曾元儿[1] 徐敏[2] 谢金华[3] 麦惠环[1] 

机构地区：[1]广州中医药大学中药学院,广州510405 [2]香港浸会大学 [3]广州中医药大学国际学院,广州510405

出　　处：《广州中医药大学学报》2004年第3期213-214,共2页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省科技计划项目 (编号 :ZKM0 490 2S)

摘　　要：【目的】通过测定骨疏灵胶囊中异补骨脂素的含量以控制制剂内在质量。【方法】采用高效液相色谱法 ,以甲醇 水(V甲醇∶V水 =4 8∶5 2 )为流动相 ,流速 1 1mL/min ,色谱柱为C18柱 ,检测波长为 2 4 5nm。【结果】异补骨脂素在 0 0 342～0 2 736 μg线性关系良好 (r=0 9998) ,平均回收率为 92 11% (RSD =1 89% ,n =5 ) ,方法重现性好 (RSD =2 0 5 % )。【结论】以高效液相色谱法检测骨疏灵胶囊中的异补骨脂素含量 ,可避免制剂中其他化学成分的干扰 ,专属性好 ,且操作简便。To determine the content of isopsoralen in Gushuling Capsule. High performance liquid chromatography was performed on C 18 column. The chromatographic conditions were as follows: methanol-water (48∶52) as mobile phase, flow rate being 1.1?mL/min and the detecting wavelength at 245?nm. A good linearity of isopsoralen was shown in the range of 0.0342～0.2736?μg, r=0.9998. The average recovery is 92.11%, RSD=1.89% (n=5). The method is with good reproducibility,RSD=2.05%.[Conclusion]This method can supply evidence for the quality control of Gushuling Capsule.

关 键 词：骨疏灵胶囊 异补骨脂素 含量测定 中药 中医 

分 类 号：R286[医药卫生—中药学]