通痹灵对胶原诱导型关节炎大鼠软骨及其细胞代谢的影响  

Effect of tongbiling prescription on cartilage and cellular metabolism in rat with collagen-induced arthritis

作　　者：陈纪藩[1] 赵诗哲[1] 陈光星[1] 刘清平[1] 陈宗良[1] 

机构地区：[1]广州中医药大学第一附属医院六内科,510405

出　　处：《中国药物与临床》2004年第5期365-368,i001,共5页Chinese Remedies & Clinics

基　　金：国家自然科学基金重点资助项目(39930230);广东省重点攻关资助项目(2003);广东省中医药管理局资助项目(102019)

摘　　要：目的探讨中药通痹灵(TBL)抗软骨破坏的作用机制。方法以胶原诱导型关节炎(CIA)大鼠为动物模型,分组灌胃治疗后,做大鼠趾关节病理切片,常规HE染色,观察软骨病变;培养软骨细胞,以鼠重组白细胞介素-1β(rrIL-1β)体外刺激软骨细胞导致软骨细胞基质降解作为药理模型,加用不同浓度的通痹灵总碱,并以地塞米松作为对照组,培养48h后,收集细胞上清液,用阿利新蓝法检测葡糖胺聚糖(GAG)含量;用硝酸酶还原法检测NO的含量。结果①趾关节病理HE切片评价结果显示:通痹灵总碱组软骨破坏程度小于造模组(P<0.05〉;②不同浓度的rrIL-1β(1、10、100、500、1000ng/ml)促进了软骨细胞蛋白多糖的降解(P<0.01),且呈量效关系;③不同浓度的通痹灵总碱(200、100、50、25mg/L)及地塞米松可明显地抑制软骨细胞蛋白多糖的降解(P<0.01)及NO的产生(P<0.01)。结论通痹灵总碱可抑制CIA大鼠趾关节软骨破坏;体外实验结果显示通痹灵总碱可对抗软骨细胞蛋白多糖的降解,其作用途径可能是通过抑制NO的生成来实现的。Objective To explore the mechanism of tongbiling (TBL) prescription for counteracting cartilage destruction.Methods After collagen-induced arthritis rats as model were medicated by gavage,pathological section of joint was made and stained with HE,then the destructions of cartilage were observed under the microscope;rat cartilage cell was cultured and stimulated with rrIL-1 at different concentrations,and proteinglycan was measured by the amount of glycosaminoglycan(GAG) released into media with Alcian blue method;after chondrocyte was incubated in the presence of rrIL-1 plus tongbiling (TBL) total alkaloids (TA) at different concentrations,and rrIL-1 plus dexamethasone as a contrast group for 48h,GAG that released into the media was determined by Alcian blue method;nitric oxide(NO) accumulation in the cell supernatant was measured with Griess reaction.Results Cartilage destructions in TA group were inferior to those in model group ;rrIL-1 of different concentrations (1,10,100,500,1000 ng/ml)induced the proteinglycan degradation in a dose-effect manner(P<0.01);TA of different concentrations (200,100,50,25 mg/L)and dexamethasone inhibited the proteinglycan degradation and NO production (P<0.01).Conclusion TA of TBL can counteract the cartilage destruction in rat with collagen-induced arthritis,and TA can inhibit the proteinglycan degradation significantly,which achieved by blocking the NO production.

关 键 词：通痹灵 胶原诱导型 关节炎 大鼠 软骨细胞 细胞代谢 重组白细胞介素-1β 药理学 

分 类 号：R285[医药卫生—中药学]