芸苔EPSPs基因cDNA的克隆和表达载体的构建  

The Cloning and Analysis of the cDNA of EPSPs Gene from Brassica campestris

作　　者：游大慧[1] 骞宇[1] 王健美[1] 郭经宇[1] 杨毅[1] 李旭锋[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2004年第3期661-664,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省"十五"育种攻关项目

摘　　要：作者从芸苔(Brassicacampestris)中用RT PCR方法获得了EPSPs基因的cDNA 与其他物种中的EPSPs基因进行了比对和分析发现:芸苔EPSPs基因的cDNA与欧洲油菜的同源性最高,为93%,与水稻同源性最低,仅为64% 将芸苔EPSPs的ORF片段插入到GTK融合表达载体中。The cDNA of EPSPs is cloned and sequenced by RT-PCR from Brassica campestris.It is blasted with the EPSPs cDNA of other 8 organisms and it is found that the EPSPs cDNA from B.Campestris is most similar with that from B.napus, and least similar with that from O.sativa. The homology of EPSPs from B.Campestris and B.napus is 93%, and only 64% from B.Campestris and O.sativa. And also, the EPSPs cDNA is inserted into an expression vector,GTK, which is derived from the pGEX-2T, the product of Pharmacia.

关 键 词：EPSPs基因 芸苔 草甘膦 RT-PCR 

分 类 号：Q785[生物学—分子生物学]