DHCC受体的纯化  

Pruification of the receptor for 1α, 25-dihydroxyvitamin D_3 form rabbit intestine

作　　者：孙宝栋[1] 石育原[1] 

机构地区：[1]白求恩医科大学分子生物学中心实验室

出　　处：《白求恩医科大学学报》1993年第5期501-504,共4页Journal of Norman Bethune University of Medical Science

基　　金：国家自然科学基金

摘　　要：本文报告了正常雄性家兔小肠粘膜细胞核提取液,经 38％饱和废硫酸铵沉淀、DEAE-纤维素层析、DNA-纤维素层析、羟基磷灰石层析、Sephadex G—200凝胶过滤,最终得到纯化5200倍的DHCC受体40μg,产率为9％。The technique in use in the purification of the rabbit intestinal receptor for 1, 25 (OH2) D3 included ammonium sulfate precipitation, DEAE-cellulose, DNA - cellulose, hydroxylapatite and Sephadex G -200 gel-exclusion Chromatography. This kind of purification scheme resulted in purification of the receptor protein by 5200 - fold with 9% yield.On analysis, the purified protein on polyacrylamide gel electrophoresis contains sodium dodecyl sulfate. Isoelectric focusing and isotachophoresis suggested that the protein is homogeneous and characterized by having a molecular weight of approximately 65 000 and a PI of 6.1.

关 键 词：分离 提纯 受体 维生素D DHCC 

分 类 号：R977.24[医药卫生—药品]