APP17肽和救脑益智胶囊水提液对D-半乳糖脑老化小鼠海马PP-1表达的影响  

THE EFFECT OF APP17 AND JIUNAOYIZHI CAPSULE ON MODULATION OF PP-1 EXPRESSIONS IN HIPPOCAMPAL NEURONS OF D-GALACTOSE INDUCED BRAIN AGING OF THE MICE

作　　者：王蓬文[1] 姬志娟[2] 杨芳 赵志炜[2] 盛树力[2] 

机构地区：[1]首都医科大学病理学教研室,北京100054 [2]首都医科大学宣武医院北京脑老化研究实验室,北京100053 [3]大兴县医院内科,北京102600

出　　处：《神经解剖学杂志》2004年第3期287-290,共4页Chinese Journal of Neuroanatomy

基　　金：北京市科学基金重点 ( 70 3 10 0 3 )资助项目

摘　　要：为探讨 APP17肽及救脑益智胶囊水提液对 D-半乳糖脑老化小鼠海马蛋白磷酸酯酶 -1( PP-1)表达的影响。本研究将昆明小鼠随机分为 5组 :正常对照组、D-半乳糖对照组、APP17肽治疗组、小剂量中药治疗组、大剂量中药治疗组 ,每组动物 8只。用 D-半乳糖制备脑老化模型 ,并皮下注射 APP17肽或救脑益智胶囊水提液灌胃对 D-半乳糖脑老化小鼠进行治疗 ,3个月后取脑组织做 PP-1免疫组织化学染色。结果显示 :D-半乳糖小鼠和大剂量救脑益智胶囊水提液治疗组小鼠海马 PP-1阳性细胞数目少 ,染色淡 ;而正常小鼠、APP17肽保护和救脑益智胶囊水提液小剂量治疗的 D-半乳糖小鼠海马阳性反应神经元数目多 ,胞浆与突起深染。结果提示 :抑制凋亡的 PP-1在 D-半乳糖小鼠海马表达降低。 APP17肽和小剂量救脑益智胶囊水提液治疗能影响 D-半乳糖脑病小鼠脑内 PP-1的表达 ,使之接近正常。The study was to investigate the effect of the peptide APP17 and the liquid extract of Jiunaoyizhi capsule on regulating the expression of protein phosphatase-1 (PP-1) in hippocampal neurons of the aging mice induced by the D-galactose(D-gal). D-gal mouse models were established by injection of D-gal. In experimental groups, mice were injected with APP17 peptide subcutaneously, or were given 0.3 g/kg or 1.0 g/kg liquid extract of raw herbs orally. After 3 months of survival, the mice were killed and their brains were cut into sections. The immunohistochemical staining of these sections was then performed with PP-1 antibody. PP-1 positive cells in the hippocampus were poorly stained in the D-gal mice. In contrast, PP-1 positive neurons were widely distributed in the hippocampus of those normal mice, the APP17 peptide-treated D-gal mice and the 0.3 g/kg liquid extract of raw herb-treated D-gal mice. These cells were darkly stained in cytoplasm. The unexpected result was that in the 1.0 g/kg liquid extract of raw herb-treated D-gal mice the number of PP-1 positive neurons did not increase to normal. The results demonstrated that the expression of PP-1 decreased in the hippocampus of D-gal mice. The APP17 and small dose liquid extract of raw herbs can regulate the distribution of PP-1 in the brain of D-gal mice and return them to normal situation.

关 键 词：淀粉样肽前体蛋白17 左旋-半乳糖老化 蛋白磷酸酯酶-1 中药 免疫组织化学 小鼠 海马 

分 类 号：R965[医药卫生—药理学]