APP17肽对IRS-1在糖尿病小鼠脑内分布的影响  

THE EFFECT OF APP17 PEPTIDE ON IRS-1 DISTRIBUTION IN BRAIN TISSUES OF DIABETIC MICE

作　　者：王蓬文[1] 姬志娟[2] 杨芳[2] 盛树力[2] 晋志高[3] 陶之理[3] 

机构地区：[1]首都医科大学病理学教研室,北京100054 [2]宣武医院脑老化研究室,北京100053 [3]中国中医研究院针灸研究所,北京100700

出　　处：《神经解剖学杂志》2004年第3期267-270,共4页Chinese Journal of Neuroanatomy

基　　金：北京市科学基金重点 ( 70 3 10 0 3 )资助项目

摘　　要：为了观察 APP17肽对糖尿病小鼠胰岛素受体底物 -1( IRS-1)的影响 ,本研究用链脲佐菌素诱发小鼠糖尿病模型 ,并皮下注射 APP17肽 ( β-淀粉样肽前体蛋白 3 19～ 3 3 5 )给予治疗 ,4周后取脑组织进行 IRS-1免疫组化染色。结果显示 ,糖尿病组IRS-1阳性反应细胞广泛分布于皮层、海马、丘脑、下丘脑等部位 ,而正常对照组及 APP17肽治疗组仅在皮层、海马见有阳性反应细胞 ,且着色淡。以上结果表明 :糖尿病小鼠脑内多个区域的神经元存在较多的 IRS-1阳性细胞 ,APP17肽能使 IRS-1阳性反应细胞出现的部位和数量正常化 。To study the effect of APP17 peptide on the distribution of IRS-1 in brain tissues of diabetic mice, mouse diabetic model was produced with streptozotocin. The diabetic mice were treated by subcutaneously injection of APP17 peptide (β-amyloid precursor protein 319-335). Four weeks later, fixative was injected intravascularly into the mice. Brain was removed and crystat sections were prepared. Immunohistochemical staining was done for IRS-1. The results demonstrated that in the brains of diabetic mice positive IRS-1 reacting neurons were numerous, darkly stained, and widely distributed in the cortex, hippocampus, and thalamus et al, while in normal mice and APP17 peptide-treated diabetic mice the positive cells were scarce and poorly stained. The present results suggest that IRS-1 was widely distributed in the brains of diabetic mice, while APP17 peptide can normalize the expression of IRS-1 and may inhibit the degeneration of diabetic mice hippocampal neurons.

关 键 词：APP17肽 IRS-1 糖尿病 小鼠 脑 胰岛素受体底物-1 神经元 

分 类 号：R587.1[医药卫生—内分泌] R338[医药卫生—内科学]