转基因烟草表达高山被孢霉Δ~6-脂肪酸脱氢酶基因的研究  

Study on the Expression of Δ~6-Fatty Acid Desaturase Gene of Mortierella alpina in Transgenic Tobacco

作　　者：李明春[1] 刘莉[1] 胡国武[1] 财音青格乐[1] 邢来君[1] 

机构地区：[1]南开大学微生物系,天津300071

出　　处：《作物学报》2004年第6期618-621,共4页Acta Agronomica Sinica

基　　金：国家自然科学基金项目 ( 3 0 2 0 0 176);高等学校骨干教师资助计划项目

摘　　要：将高山被孢霉ATCC16 2 6 6Δ6 脂肪酸脱氢酶 (D6D)基因亚克隆到植物表达载体pBI12 1中 ,构建了CaMV35S启动子驱动的植物表达载体pBMACL6。经根癌农杆菌LBA4 4 0 4的介导 ,采用叶盘法转化烟草Xanthi,得到一批转基因烟草植株。转基因植株的PCR和Southernblot分析表明 ,D6D基因已经整合到烟草基因组中。脂肪酸GC分析表明 ,与未转基因的烟草苗对照相比 ,转基因植株有D6D基因目标产物γ 亚麻酸的产生 ,说明高山被孢霉的D6D基因在烟草中得到了表达。The plant transformation vector pBMACL6 was constructed to express Δ 6 fatty acid desaturase (D6D) gene of Mortierella alpina cDNA under control of the CaMV35S promoter. Tobacco was transformed by co cultivating leaf dics with Agrobaterium strains harboring pBMACL6. The regenerated Kanamycin resistant transformants were analyzed by PCR, Southern blot and GC analysis of fatty acids. These results indicated the D6D gene integrated into the tobacco genomic DNA and expressed efficiently. γ linolenic acid (18∶3 Δ 6,9,12 , GLA) and a new fatty acid, Octadecatetraenoic acid(18∶4 Δ 6,9,12,15 , OTA) was detected in all transgenic tobacco.

关 键 词：高山被孢霉△^6-脂肪酸脱氢酶基因 转基因烟草 Γ-亚麻酸 品质改良 油料作物 

分 类 号：S336[农业科学—作物遗传育种]