氯化硝基四氮唑蓝共振散射法检测DNA  

Determination of Deoxyribonucleic Acid with Nitro-Tetrazolium Chloride Blue by Resonance Light-Scattering Technique

作　　者：龙云飞[1] 陈小明[1] 蔡铁军[2] 

机构地区：[1]湘潭大学化学学院,湖南湘潭411105 [2]湘潭师范学院化学系,湖南湘潭411201

出　　处：《光谱学与光谱分析》2004年第5期610-612,共3页Spectroscopy and Spectral Analysis

基　　金：湖南省自然科学基金(00JJY2084);湖南省教育厅(03C611)资助项目

摘　　要：研究了氯化硝基四氮唑蓝与脱氧核糖核酸在酸性条件下的共振光散射特征 ,考察了影响因素和最佳反应条件 ,建立了一种测定DNA的新方法。在pH =1 8三羟甲基氨基甲烷 (Tris HCl)缓冲溶液中 ,线性范围为 0～ 7mg·L-1,相关系数为 0 995 8,检出限为 30 0 4ng·mL-1,用于合成样品的测定结果令人满意。Characteristics of the interaction of nitro-tetrazolium chloride blue and Deoxyribonucleic Acid(DNA) were studied in the of pH 1.8 buffer solution by Resonance Light-Scattering (RLS) technique. The effects of interference substances and the optimization of the procedure were investigated, and a new method for the determination of the DNA was established based on RLS. The linear range of the DNA is 0-7 mg(.)L(-1) with a correlation coefficient of 0.995 8. The detection limit is 30.04 mug(.)L(-1). The method has been applied to the determination of DNA in mixed samples with satisfactory result.

关 键 词：氯化硝基四氮唑蓝 共振散射光谱 DNA 脱氧核糖核酸 

分 类 号：Q523[生物学—生物化学]