非线性偏最小二乘导数分光光度法同时测定复方新诺明中二组分的含量  

Nonlinear Partial Least Squares Method for Simultaneous Derivative Spectrophotometric Determination of Two Components in Compound Sulfamethoxazole

作　　者：周彤[1] 钟家跃[1] 冯江[1] 

机构地区：[1]江西医学院预防医学系,江西南昌330006

出　　处：《光谱学与光谱分析》2004年第5期616-618,共3页Spectroscopy and Spectral Analysis

基　　金：江西省自然科学基金(010006)资助项目

摘　　要：构造了导数光谱与多组分浓度间的非线性模型 ,以偏最小二乘法估计模型参数 ,建立了同时测定两组分的非线性偏最小二乘导数分光光度法 ,方法用于复方新诺明中磺胺甲唑SMZ(Sulfamethoxazole)和甲氧苄啶TMP(Trimethoprim)含量的测定 ,10份模拟样中SMZ ,TMP的回收率分别为 99 8%和 10 0 1% ,RSD分别为 1 3%和 1 6 %。结果明显好于线性偏最小二乘导数分光光度法。用该法测定复方新诺明片中的二组分含量测定结果与药典方法一致 ,该法为复方制剂的分光光度分析提供了更为理想的新途径。A nonlinear model of consistency with derivative absorption in multiple components systems has been given. Model data were estimated by partial least squares method. A method for the simultaneous derivative spectrophotometric determination of two components with nonlinear partial least squares method was established. The method has been applied to the simultaneous determination of two components in compound sulfamethoxazole tablets. The average recoveries of sulfamethoxazole and trimethoprim in synthetic samples were 99.8% and 100.1%, and relative standard deviations were 1.3% and 1.6%, respectively. The results by nonlinear PLS method are significantly better than those by linear PLS method. The results for actual compound formulation agreed with those obtained by standard method.

关 键 词：非线性偏最小二乘法 导数分光光度法 复方新诺明 含量测定 同时测定 磺胺甲噁唑 甲氧苄啶 

分 类 号：R927.2[医药卫生—药学]