腰带长体茧蜂寄生对亚洲玉米螟幼虫体内酚氧化酶活性的影响  

Effects of parasitism by Macrocentrus cingulum Brischke (Hymenoptera: Braconidae) on the activity of phenoloxidase in larvae of the Asian corn borer, Ostrinia furnacalis Guenée (Lepidoptera: Pyralidae)

作　　者：冯从经[1] 邱鸿贵[1] 邱中良[1] 符文俊[1] 

机构地区：[1]中国科学院上海生命科学院植物生理生态研究所,上海200025

出　　处：《昆虫学报》2004年第3期298-304,共7页Acta Entomologica Sinica

基　　金：国家自然科学基金重点项目 (3 993 0 0 3 0 )

摘　　要：通过对被腰带长体茧蜂MacrocentruscingulumBrischke寄生的 5龄亚洲玉米螟OstriniafurnacalisGuen啨ｅ幼虫体内不同组织中酚氧化酶活性的测定 ,采用体外注射腰带长体茧蜂雌性成蜂的萼液成分、毒液成分、萼液与毒液混合物的方法 ,研究了寄生蜂各种主要生理因子对寄主血清中酚氧化酶活性的影响。结果表明 :寄生蜂寄生可明显抑制寄主体内的酚氧化酶活性 ,减少黑色素产生 ;被寄生组FITC标记的血细胞阳性百分率低于未被寄生组 ,差异极显著 (P <0 0 1) ;萼液成分可明显地抑制亚洲玉米螟幼虫血清中酚氧化酶的活性 (P <0 0 1) ;萼液与毒液混合物对酚氧化酶活性也有明显抑制作用 (P <0 0 1)。研究认为寄生蜂产卵时注入的萼液、毒液可对寄主昆虫酚氧化酶活性产生明显的抑制作用 ,其中萼液是抑制寄主免疫能力的主要因素。The activity of phenoloxidase in various tissues of Ostrinia furnacalis Guene fifth-instar larvae parasitized by Macrocentrus cingulum Brischke was investigated. The effects of various physiological factors on the activity of phenoloxidase and melaninization in the hemolymph of O. furnacalis larvae were examined by the injection of calyx fluid, venom, the mixture of calyx fluid plus venom of M. cingulum in vitro. The results showed that the activity of phenoloxidase in hemolymph of O. furnacalis was inhibited significantly by parasitism and the injection of calyx fluid or the mixture of calyx fluid plus venom of M. cingulum (P<0.01), and the production of melanin was also reduced. The percentage of positive FITC-labelled hemocytes of the parasitized host larvae was lower than that of unparasitized larvae (P<0.01). It was concluded that the melaninization could be inhibited by the injected the calyx fluid, venom or other components produced by the parasitic wasp along with laying eggs, in which calyx fluid was probably the main factor inhibiting the ability of immunity of host insect.

关 键 词：腰带长体茧蜂 亚洲玉米螟 寄生 萼液 酚氧化酶 

分 类 号：Q965[生物学—昆虫学]