桃蛀果蛾寄主生物型分化的RAPD分析  

RAPD analysis on the speciation in host races of Carposina sasakii Matsumura (Lepidoptera: Carposinidae)

作　　者：徐庆刚[1,2] 花保祯[1] 

机构地区：[1]西北农林科技大学植物保护学院,教育部植保资源与病虫害治理重点实验室,陕西杨凌712100 [2]江苏大学生物与环境工程学院生命科学研究院,江苏镇江212013

出　　处：《昆虫学报》2004年第3期379-383,共5页Acta Entomologica Sinica

基　　金：国家自然科学基金项目 (3 95 70 0 95 )

摘　　要：以山茱萸蛀果蛾CarposinacoreanaKim为外群 ,用 4 4个随机引物对苹果、山楂、桃、杏、枣和酸枣等 6种寄主植物上的桃蛀果蛾CarposinasasakiiMatsumura进行了RAPD分析。结果表明 ,不同寄主上的桃蛀果蛾之间遗传差异出现了较大的分化 ,桃蛀果蛾 6个寄主生物型之间的平均遗传距离在 0 0 4 0～ 0 6 37之间 ,尤其是杏生物型分化最大。经聚类分析 ,可将不同寄主上的桃蛀果蛾明显分成两个类群 ,杏生物型为一群 ,其余为一群。两个类群间遗传距离为 0 4 6 5 ,亚群内部的遗传距离为 0 0 4 0～0 36 9。推测杏树上的“桃蛀果蛾”已经分化成为不同的物种。With Carposina coreana Kim as the out-group, using 44 random primers,the RAPD analysis was conducted on the host races of the peach fruit borer, Carposina sasakii Matsumura, from 6 different host plants, i.e., apple, hawthorn, peach, apricot, jujube and wild jujube, in Shaanxi Province. The results show that there exists great genetic differentiation between the host races. The genetic distances among the six host races of the peach fruit borer vary from 0.040 to 0.637, with the apricot race differs most markedly from other host races. Based on the clustering analysis, the six host races of the peach fruit borer from the various host plants can be divided into two sub-groups: the apricot race as a sub-group, and the others as another sub-group. The genetic distance is 0.465 between the two sub-groups, and 0.040 to 0.369 within a sub-group. It is suggested that the apricot race of the peach fruit borer have been so differentiated genetically with the others that it should be considered as a good species.

关 键 词：桃蛀果蛾 山茱萸蛀果蛾 物种分化 生物型 RAPD 

分 类 号：Q965[生物学—昆虫学]