提高腺病毒载体转染造血细胞效率的研究进展  

Progress of Studies on Enhancing Efficiency of Gene Transfection into Hematopoietic Cells with the Adenoviral Vector —— Review

作　　者：王莉莎[1] 段海峰[1] 王立生[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《中国实验血液学杂志》2004年第3期383-386,共4页Journal of Experimental Hematology

摘　　要：重组腺病毒作为基因转移的载体已经广泛应用于基因治疗的基础研究和临床试验 ,但造血细胞由于缺乏腺病毒特异性受体柯萨奇 腺病毒受体 (coxsackevirusandadenovirusreceptor ,CAR)导致转染效率较低 ,从而限制了腺病毒载体在造血系统的应用。多种策略可以提高腺病毒转染造血细胞的效率 ,这些策略包括腺病毒本身的改造、造血细胞CAR表达水平的提高等。本文就这一领域的主要研究进展作一综述。Recombinant adenoviral vectors have been widely applied for the basic research and clinical trials of gene therapy. However,the inability of adenovirus to infect hematopoietic cells which lack the specific adenovirus receptors - coxsackie virus and adenovirus receptor (CAR) represents an important limitation in therapeutic applications. This limitation may be overcome by several approaches including modification of adenovirus vector and improvement of the susceptibility of hematopoietic cells. The current progresses in this field were summarized.

关 键 词：腺病毒载体 基因治疗 造血细胞 

分 类 号：Q782[生物学—分子生物学] R331.2[医药卫生—人体生理学]