VEGFA、VEGFC及其受体和angiopoietin-1/angiopoietin-2及其受体在人胚胎卵黄囊血岛、AGM区的表达  

Expression of VEGFA,VEGFC,Angiopoietin-1,Angiopoietin-2 and Their Receptors on Yolk Sac Blood Island,Aorta-Gonad-Mesonephros (AGM) Region of Human Embryo

作　　者：蒋吉英[1] 李爱冬[2] 梅妍[1] 周鸿鹰[1] 羊惠君[1] 杨淑霞[2] 洪华容 宋红瑞 

机构地区：[1]四川大学华西医学中心解剖教研室,成都610044 [2]第三军医大学成都军医学院科研实验中心

出　　处：《中国实验血液学杂志》2004年第3期249-254,共6页Journal of Experimental Hematology

摘　　要：本研究观察VEGFA、VEGFC及其受体和angiopoietin 1、angiopoietin 2及其受体在发育第 3- 12周人胚胎卵黄囊血岛、主动脉 生殖腺 中肾 (AGM )区的表达。在征得意外流产孕妇同意并签字后 ,收集其意外流产的受精龄第 3- 12周的人胚胎 ,用 4 %多聚甲醛固定 ,石蜡包埋切片 ,HE和免疫组织化学染色 ,光镜观察。结果表明 :第2 1- 2 5天人胚卵黄囊血岛血管内皮细胞和造血细胞强表达VEGFA及其受体flt1和flk 1,VEGFC及其受体flt4 ,angiopoietin 2及其受体Tie 2蛋白 ;弱表达angiopoietin 1蛋白。背主动脉和中肾于发育第 4周开始表达上述各种因子及其受体 ,免疫阳性反应物集中在大而圆、有核的造血细胞。阳性细胞的数量到第 7周到达高峰。 8周以后 ,阳性细胞数量显著减少 ,到 12周几乎所有的血细胞为免疫阴性反应。生殖腺主要在第 6 - 8周表达VEGFA ,flt1,flt4 ,angiopoietin 1,angiopoietin 2和Tie 2蛋白 ,但不表达VEGFC和flk 1。在AGM区的血管内皮细胞未检测到上述各因子的表达。结论 :人胚胎卵黄囊血岛造血细胞和内皮细胞以及背主动脉和中肾共表达多种与血管发生和血液发生相关的因子。人胚内造血发生于第 4周。The study was to investigate the expression of VEGFA,VEGFC,angiopoietin-1,angiopoietin-2 and their receptors on yolk sac blood island,AGM region during gestation of 3th-12th weeks of human embryo. Human embryo contingently aborted at 3-12 weeks of gestation were collected with signed agreements of the pregnant women suffered from accidenteal abortions. The specimens were fixed by 4% paraformaldehyde and embedded by paraffin. 5 μm serial sections were made. HE and immunohistochemistry method (SABC) and light-microscope were employed. The results showed that VEGFA and its receptors flt1/flk-1,VEGFC and its recepter flt-4,angiopoietin-2 and its recepter tie-2 proteins were expressed strongly and angiopoietin-1 was weakly expressed by hematopoietic cells and vascular endothelial cells of blood island at 21 and 25 days of gestation. In the 4th week of gestation,immuno-positive reaction of these factors and their receptors appeared in the aorta and mesonephros deposited in larger,rounded and nucleated cells which represented hematopoietic cells. Up to 7th week,positive hematopoietic cells in the regions were much abundant. The number of positive cells decreased at 8th week. Up to 12th week,almost all blood cells were immuno-negative. VEGFA,flt-1,flt-4,angiopoietin-1,angiopoietin-2 and Tie-2 protein were expressed mainly by gonad at 6-8 weeks,but it did not express VEGFC and flk-1. The immuno-reaction of the factors and their receptors could not detected in vascular endothelial cells during 3-12th weeks of gestation. It is concluded that hematopoietic cells and endothelial cells in blood island of yolk sac,mesonephros and dorsal aorta co-expressed some factors and their receptors in relation to vasculogenesis and hematopoiesis. Intraembryonic hematopoiesis began in the 4th week of gestation.

关 键 词：造血细胞 卵黄囊 血岛 AGM区 VEGF家族 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学] R331.2[医药卫生—基础医学]