应用气升式反应器培养草酸青霉菌菌丝球的研究  

Study on Producing Mycelium Pellets of Penicillium oxalicum in An Airlift Reactor

作　　者：袁丽梅[1,2] 张书军[1] 杨敏[1] 杨清香[1] 章丽萍[3] 

机构地区：[1]中国科学院生态环境研究中心 [2]中国矿业大学化工与环境工程学院北京100083 [3]中国矿业大学化工与环境工程学院

出　　处：《微生物学报》2004年第3期393-395,共3页Acta Microbiologica Sinica

基　　金：国际合作项目 ;日本国土环境株式会社资助~~

摘　　要：应用 0 5L气升式反应器成功实现了对高效染料吸附丝状真菌———草酸青霉菌 (Penicilliumoxalicum)的颗粒化 ,初步解决了菌体与培养液分离的问题。以OD6 0 0 为 0 2 3的孢子悬浊液接种时 ,菌丝成球的最佳接种量为2 5 % ,最佳通气量为 0 5L min。菌球直径及沉降速度随时间均呈线性增长。在最佳接种量 (2 5 % )和最佳通气量(0 5L min)下 ,30℃培养 72h后 ,菌球的直径和沉降速度分别为 (4 0± 0 8)mm和 (1 4± 3)mm s。The mycelium pellets of high-performance dye-adsorbing filamentous fungus, Penicillium oxalicum were successfully produced in a 0 5L airlift reactor. The pellets were compact and could settle rapidly, which facilitate the recovery of the pellets from culture. The optimal inoculum was 2 5% and the optimal air rate was 0 5L/min under a spore inoculum OD 600 of 0 23. Both diameters and settling rates of the pellets increased linearly with the increase of culture time. The diameter and settling rate of the pellets in de-ionized water were(4 0±0 8)mm and(14±3)mm/s, respectively, following culture in the optimal conditions (inoculum: 2 5%; air rate: 0 5L/min;30℃) for 72h.

关 键 词：菌丝球 气升式反应器 青霉菌 吸附 

分 类 号：X788[环境科学与工程—环境工程]