活血补肾中药对培养成骨细胞VEGF活性的影响  

Effects of Danxian Kanggu capsule on the vascular endothelial growth factor expression and production in osteoblasts

作　　者：沈冯君[1] 刘日光[1] 杨述华[2] 沈骏[1] 易诚青[2] 李新春[2] 刘建湘[2] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]华中科技大学同济医学院协和医院骨科

出　　处：《中国骨伤》2004年第5期260-262,共3页China Journal of Orthopaedics and Traumatology

基　　金：贵州省自然科学基金资助项目 [计字 (2 0 0 1 ) 30 4 9]

摘　　要：目的 :研究活血补肾中药丹仙康骨胶囊对体外培养成骨细胞VEGF活性的影响 ,进一步探讨丹仙康骨胶囊抗骨坏死的作用机制。方法 :应用RT -PCR及流式细胞仪检测观察不同剂量丹仙康骨胶囊对成骨细胞VEGFmRNA的表达与VEGF的生成的影响。结果 :丹仙康骨胶囊可以促进成骨细胞VEGFmRNA的表达与VEGF的生成 ,并呈剂量依赖性。结论 :丹仙康骨胶囊促进成骨细胞VEGFmRNA的表达与VEGF的生成可能是其抗骨坏死的作用机制之一。Objective:To investigate the effects of Danxian Kanggu capsules extracted from traditional Chinese medicine on the vascular endothelial growth factor expression and production by osteoblats,and explore the mechanism of anti-osteonecrosis of Danxian Kanggu capsules.Methods:Osteoblasts derived from fetal rats were used in this study.VEGF mRNA expression,intracellular production induced by Danxian Kanggu capsules were measured by RT-PCR and flow cytometry.Results:Danxian Kanggu capsules could significantly increase vascular endothelial growth factor expression and production in osteoblats in a dose-dependent manner.Conclusion:The anti-osteonecrosis effect of Danxian Kanggu capsules may be through its increasable effect on vascular endothelial growth factor expression and production in osteoblasts.

关 键 词：活血补肾中药 成骨细胞 VEGF活性 血管内皮生长因子 丹仙康骨胶囊 

分 类 号：R269.81[医药卫生—中西医结合] R28[医药卫生—中医外科学]