儿茶素对骨髓细胞周期及造血生长因子基因表达的作用  

Effect of catechin on bone marrow cell cycle and gene expression of hematopoietic growth factors

作　　者：刘屏[1] 王东晓[1] 陈若芸[2] 陈孟莉[1] 殷建芬[1] 陈桂芸[1] 

机构地区：[1]中国人民解放军总医院临床药理研究室,北京100853 [2]中国医学科学院,中国协和医科大学药物研究所,北京100050

出　　处：《药学学报》2004年第6期424-428,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 890 )

摘　　要：目的　探讨中药鸡血藤SpatholobussuberectusDunn活性成分儿茶素对小鼠骨髓细胞增殖周期及其脾细胞内造血生长因子IL 6和GM CSFmRNA表达的影响 ,以初步阐明其造血调控作用机理。方法　用流式细胞仪检测技术观察儿茶素对正常及骨髓抑制小鼠骨髓细胞增殖周期的影响 ,同时采用RT PCR技术 ,检测在儿茶素刺激条件下 ,小鼠脾细胞内IL 6和GM CSFmRNA表达的变化。结果　儿茶素可促使正常及骨髓抑制小鼠骨髓细胞G0 G1期细胞比例下降 ,S +G2 M期细胞比例增加 ,并可使正常及骨髓抑制小鼠脾细胞内IL 6mRNA和GM CSFmRNA表达显著上调。结论　儿茶素可通过诱导脾细胞内IL 6mRNA和GM CSFmRNA的表达 ,促进正常小鼠骨髓细胞进入增殖周期 ,并促使骨髓抑制小鼠的骨髓细胞跳出“G1 期阻滞” ,进入细胞增殖周期 ,从而加速造血干 祖细胞的增殖和分化。Aim To study the effect of catechin, the active component of Spatholobus suberectus Dunn, on bone marrow cell cycle and the expression of IL 6 and GM CSF mRNA in spleen cells of normal and marrow depressed mice in order to clarify the mechanism of hematopoietic supportive effect of catechin. Methods Flow cytometry was adopted to investigate the influence of catechin on bone marrow cell cycle in mice and the expression of IL 6 and GM CSF mRNA induced by catechin in spleen cells was detected by RT PCR technique simultaneously. Results The cell proportion of normal and marrow depressed mice in G 0 /G 1 phase was reduced significantly, while that in S+G 2 /M phase increased significantly. Being induced by catechin, IL 6 mRNA and GM CSF mRNA in spleen cells were markedly up regulated. Conclusion Catechin (2 g·L -1 , intraperitoneally injected to mice daily immediately after irradiation for 7 consecutive days) was shown to promote the expression of IL 6 mRNA and GM CSF mRNA in spleen cells of mice, through which it can accelerate bone marrow cells of normal mice into cell cycle and help those of marrow depressed mice to get out of “G 1 phase block”, enter into cell cycle and radically accelerate the proliferation and differentiation of hematopoietic stem cell/hematopoietic progenitor cell (HSC/HPC).

关 键 词：儿茶素 骨髓细胞 造血生长因子 基因表达 细胞周期 中药 鸡血藤 

分 类 号：R285.5[医药卫生—中药学]