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出 处:《动物学杂志》2004年第3期8-11,共4页Chinese Journal of Zoology
基 金:国家"973"计划资助项目 (No.G1 9990 1 2 0 0 5 ) ;山东省中青年学术骨干资助项目
摘 要:以阿新兰和过碘酸雪夫氏反应 (AB PAS)染色法显微观察皱纹盘鲍 (Haliotisdiscushannai)的外套膜、鳃和足的粘液细胞。根据所显示颜色的不同 ,可将粘液细胞分为Ⅰ~Ⅳ 4种类型 :分别呈红色、蓝色、紫红色和蓝紫色。外套触手和外套膜上皮的粘液细胞以Ⅱ型为主 ,Ⅳ型较少 ,密度不均 ,多为近圆形细胞 ,大型和小型细胞均有分布。鳃轴和鳃叶上皮的粘液细胞密度较大 ,以Ⅱ型和Ⅰ型为主 ,Ⅲ型和Ⅳ型较少 ,形态有杯形、近圆形或棒状等 ,多为中型及小型细胞。足的上皮粘液细胞较少 ,均为Ⅱ型 。Mucous cells in the mantle,gill and foot of abalone Haliotis discus hannai were observed and analyzed by the method of AB-PAS (alcian blue and periodic acid schiffs reaction) combined procedure.According to the color of the mucous cells,they were divided into four types:typeⅠ,pure red; typeⅡ,pure blue; type Ⅲ,purple reddish; type Ⅳ,blue purple.Most of the mucous cells in the epithelia of mantle and tentacle of mantle were in type Ⅱ and a few of them were in type Ⅳ.The shapes of most mucous cells was in circle.The distribution of the mucous cells was not uniform in the tissues.The mucous cells were rich in the epithelia of gill axis and gill filaments,and most of them were in type Ⅱ and type Ⅰ,while a few were in type Ⅲ and type Ⅳ. Their shapes were cup-,circle- and stick-like.There were a few mucous cells in the epithelia of the foot,and all of them were in type Ⅱ.There were many brown particulates in some of the epithelial cells of the foot.
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