冷驯化条件下长爪沙鼠血清瘦素浓度的变化及其与能量收支和产热的关系  

Effects of cold acclimation on body weight, serum leptin level, energy metabolism and thermogenesis in the Mongolian gerbil Meriones unguiculatus

作　　者：李兴升[1] 王德华[1] 杨明[1] 

机构地区：[1]中国科学院动物研究所,农业虫鼠害综合治理研究国家重点实验室,北京100080

出　　处：《动物学报》2004年第3期334-340,共7页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金项目 (No .3 0 170 15 1;3 9970 12 8);中国科学院创新工程战略方向和领域前沿项目 (No .KSCX2 SW 10 3和KSCX2 1 0 3 );中国科学院生物科学与生物技术研究特别支持费项目 (No .STZ 0 1 0 6)资助~~

摘　　要：为研究低温胁迫条件下长爪沙鼠的适应对策及瘦素对体重和能量平衡的调节作用 ,我们将 7只成年雌性长爪沙鼠在 5℃条件下驯化 2 1d ,另选 7只作为对照 ,对体重、血清瘦素含量、体脂含量、摄入能、基础代谢率、非颤抖性产热等进行了测定。结果发现 :1 ) 5℃条件下长爪沙鼠的体重没有明显变化 ;2 ) 5℃条件下长爪沙鼠的血清瘦素浓度和体脂含量均明显低于对照组 ,且瘦素浓度与体脂含量呈显著正相关 ;3) 5℃条件下长爪沙鼠的摄入能、基础代谢率和非颤抖性产热等显著高于对照。这些结果表明 :长爪沙鼠在低温条件下产热能力和自身维持能量消耗都增加 ,能量摄入因此而增加 ;瘦素参与了能量平衡和体重的调节 。To determine the adaptive strategy and the role of leptin in the regulation of body weight and energy balance, body weight, serum leptin level, body fat mass, energy intake, basal metabolic rate and nonshivering thermogenesis were measured after 3 weeks acclimation at 5℃ for adult female Mongolian gerbils Meriones unguiculatus. Body mass was monitored every 3 days. Body fat content was analysed with the Soxhlet method. Energy intake was measured in food trials. Metabolic trials were conducted with closed circuit respirometer and nonshivering thermogenesis was induced by injection of mass dependent dosage of noradrenaline. Serum leptin concentration was measured with a commercial multispecies leptin RIAkit (Linco Company, USA). Results showed that: 1) no significant changes in body weight were found at 5℃; 2) serum leptin levels and body fat mass were lower at 5℃ than that of control (23℃), there is a positive correlation between leptin concentration and body fat mass; 3) energy intake, basal metabolic rate, nonshivering thermogenesis were higher at 5℃ than that of control. These results suggested that Mongolian gerbils enhanced thermogenic capacity and increased their maintenance cost during cold acclimation, thus could cause the increase in energy intake. Leptin participated in the regulation of energy balance and body weight for Mongolian gerbils.However, it seemed that there was no direct relationship between serum leptin concentration and thermogenesis.

关 键 词：长爪沙鼠 体重 瘦素 能量代谢 浓度 低温胁迫 

分 类 号：Q41[生物学—生理学]