乳鼠预损伤周围神经雪旺细胞的培养及增殖能力研究  

Culture and proliferation of Schwann cells obtained from lesioned peripheral nerves of newborn rats

作　　者：黄涛[1] 秦建强[2] 王宏亮[3] 武雷[2] 杨俊[2] 余磊[2] 许忠[1] 徐如祥[1] 

机构地区：[1]解放军第一军医大学珠江医院神经外科,广东省广州市510000 [2]解放军第一军医大学临床解剖学研究所,广东省广州市510000 [3]阜阳市第一人民医院骨科,安徽省阜阳市236000

出　　处：《中国临床康复》2004年第17期3294-3295,i003,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金(39970833);广东省科技重点攻关项目(2003A3020101;2003A3020304)~~

摘　　要：目的:从乳鼠预损伤的坐骨神经中分离培养雪旺细胞。方法:预损伤SD乳鼠坐骨神经,3d后取损伤的坐骨神经,手术显微镜下分离神经外膜,用胰酶、胶原酶消化,差速贴壁除去成纤维细胞,接种培养后对细胞进行计数、MTT活性检测,并用S-100蛋白标记观察。结果:该方法所培养的雪旺细胞形态正常,数量及纯度高,增殖旺盛。结论:此实验为神经组织工程研究中雪旺细胞的来源提供了一个有效的方法。AIM:To culture Schwann cells detached from lesioned sciatic nerves of newborn rats. METHODS:Sciatic nerves of newborn SD rats were prepared and lesioned sciatic nerves were collected 3 days later. The nerve fascicles were extracted under operating microscope, digested by pancreatin and collagenase. Schwann cells were cultured in cell culture medium after eliminating fibroblast by differential anchoring. Schwann cells were counted and the cell activity was estimated with MTT method, moreover, these cells were examined by S 100 protein labelling. RESULTS:Schwann cells were morphologically normal, with highly purification and number, as well as high proliferation after culture in this way. CONCLUSION:This method provides an effective method to gain Schwann cells for nerve tissue engineering study.

关 键 词：乳鼠 周围神经损伤 雪旺细胞 细胞培养 细胞增殖 

分 类 号：R651.3[医药卫生—外科学]