C57BL/6小鼠CIA模型的建立及其监测体系的初步筛选  

Replication of collagen induced arthritis in C57BL/6 mice

作　　者：贾俊峰[1] 李晓燕[1] 朱平[1] 王彦宏[1] 樊春梅[1] 赵雯[1] 

机构地区：[1]第四军医大学西京医院临床免疫科,西安710032

出　　处：《解放军医学杂志》2004年第6期472-474,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家"8 63"计划资助课题 (编号 2 0 0 1AA2 1 50 61 )

摘　　要：目的　建立稳定的C5 7BL/ 6小鼠胶原诱导关节炎 (CIA)模型 ,构建进一步进行类风湿关节炎 (RA)发病机制及治疗研究的体系。方法　取 4 0只雄性C5 7BL/ 6小鼠 ,随机分为模型组和对照组。模型组采用鸡II型胶原与完全弗氏佐剂在尾部皮内注射 ,2 1天后同样方法加强免疫。定期观察两组小鼠的关节和关节外表现以及病理学改变 ;用三重免疫荧光标记、胞内细胞因子测定流式细胞术检测外周血T细胞亚群。结果　对照组小鼠均未发病。模型组小鼠在 5 0天的发病率达 70 % ,关节炎评分在 3～ 8分 ,持续时间大约 1 0 0天 ;病理切片显示典型的炎性细胞浸润 ,滑膜增生 ,软骨及软骨下骨破坏 ;外周血Th1 /Th2比值升高。结论　应用Ⅱ型胶原能在C5 7BL/ 6小鼠上成功建立CIA模型 。Objective To replicate a model of type Ⅱ collagen (C Ⅱ) induced arthritis in C57BL/6 mice. Methods C57BL/6 mice were immunized by intradermal injection at the base of the tail with chick type Ⅱ collagen and complete Freund adjuvant emulsion, followed by another injection 21 days later. Manifestations of joint ailment, pathological examination, and T cell subtypes detected by FCM were observed. Results In comparison with control mice, the C57BL/6 mice developed CIA with high incidence (70%) and severity after immunization. Hyperplasia of the synovium and inflammatory infiltration were observed. The percentage of Th1 cells in the peripheral blood of the mice was significantly increased in the peripheral mononuclear cells (PBMC). Conclusion The CIA model of C57BL/6 mice and the platform its study were successfully established.

关 键 词：C57BL/6小鼠 关节炎 类风湿 Ⅱ型胶原诱导的关节炎 疾病模型 动物 

分 类 号：R593.22[医药卫生—内科学] R332[医药卫生—临床医学]