盐酸博莱霉素致大鼠肺纤维化动物模型HRCT的评价  

Evaluation the diagnostic value of HRCT in a rat model of pulmonary fibrosis induced with bleomycin A5

作　　者：张东伟[1] 王继峰[1] 牛建昭[1] 高宝华[1] 赵景民[2] 周光德[2] 

机构地区：[1]北京中医药大学细胞与生化实验室,北京100029 [2]解放军第302医院

出　　处：《解放军医学杂志》2004年第6期507-509,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 30 1 30 2 2 0 )

摘　　要：目的　评价高分辨率CT(HRCT)对盐酸博莱霉素致大鼠肺纤维化动物模型的诊断及鉴别诊断价值 ,探讨能应用于放射影像学研究的肺纤维化动物模型。方法　雄性SD大鼠 6 0只 ,其中 5 0只经气管滴注盐酸博莱霉素 ,1 0只经气管滴注等体积的生理盐水。2 8天后平行观察大鼠HRCT征象 ,行HRCT 病理对照研究。结果　经气管一次性滴注盐酸博莱霉素的大鼠均出现了纤维化病变 ,HRCT可见肺实变、结节影、血管支气管束异常、胸膜增厚、交界面不规则、磨玻璃样密度影等 ,部分可见蜂窝肺 ,病理切片对照显示肺组织纤维性增生。结论　HRCT方法适于诊断活体动物肺纤维化 ,该方法方便、准确、可靠 。objective To evaluate the value of HRCT on differential diagnosis of a rat model of pulmonary fibrosis induced with bleomycin A5 and to replicate a rat model of pulmonary fibrosis for radio imaging study to investigate pathogenetic mechanism of pulmonary fibrosis. Methods In 50 of 60 male Sprague Dawley rats bleomycin A5 was given through the trachea, and the other 10 received same volume of normal saline. After 28 days, HRCT and pathological examination of the lungs were performed, and the changes were correlatively studied. Results HRCT showed pulmonary fibrosis in all the 50 rats treated with bleomyein, including ground glass opacity, nodular shadow, abnormality in bronchio vasarlar linings, pleural thickening, and in some with beehive like changes. Lung fibrosis was corroborated by pathological sections. Conclusion HRCT can be used to diagnose pulmonary fibrosis of living rats. It is a simple, accurate, reliable, and it can give good representation.

关 键 词：动物模型 大鼠 肺纤维化 盐酸博莱霉素 鉴别诊断 诊断 高分辨率CT 

分 类 号：R563[医药卫生—呼吸系统] R816.41[医药卫生—内科学]