杭白菊提取液对血管平滑肌细胞培养液SOD、MDA的影响  

Effect of Chrysanthemum Extract on Superoxide Dismutase and Malondialdehyde in Cultural Liquid of Bovine Vascular Smooth Muscle Cells

作　　者：王淑如[1] 章云涛[1] 方雪玲[1] 王锡田[1] 李霞[1] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003

出　　处：《浙江预防医学》2004年第7期13-14,共2页Zhejiang Journal of Preventive Medicine

基　　金：浙江省中医药局课题 (2 0 0 2C0 77)

摘　　要：目的　探讨杭白菊提取液对小牛主动脉平滑肌细胞 (VSMC)培养液SOD、MDA的影响。方法　取小牛主动脉进行体外VSMC培养 ,加入杭白菊提取液干预 ,取上清液在分光光度仪下测定超氧化物歧化酶 (SOD)、丙二醛 (MDA)。结果　加入杭白菊提取液后SOD值升高、MDA值下降 ,且随着杭白菊提取液剂量增加 ,作用增强。结论　杭白菊提取液具有抗氧化作用 ,并呈剂量依赖性。Objective To explore the effect of chrysanthemum extract on superoxide dismutase (SOD) and malondialdehyde (MDA) in cultural liquid of bovine vascular smooth muscle cells (VSMCs). Methods VSMCs were isolated from calf thoracic aorta and cultured in vitro, then chrysanthemum extract was added in, finally the supernatant liquid was obtained to examine SOD and MDA by spectrophotometer. Results The value of SOD increased and that of MDA decreased when chrysanthemum extract was added, which had a dose response relationship. Conclusions Chrysanthemum extract has an anti oxidation effect in a dose dependent manner.

关 键 词：杭白菊 提取液 血管平滑肌细胞 培养液SOD MDA 自由基 

分 类 号：R282.71[医药卫生—中药学] R322.12[医药卫生—中医学]