单分散N-苯甲氧羰基-L-色氨酸表面分子印迹聚合物的手性拆分  

Uniform-sized Molecularly Imprinted Polymer for N-Carbobenzoxy-L-tryptophan and Its Chromatographic Properties

作　　者：郭天瑛[1] 张丽影[1] 郝广杰[1] 宋谋道[1] 张邦华[1] 

机构地区：[1]南开大学高分子化学研究所

出　　处：《分析化学》2004年第6期705-709,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目 (No .5 0 0 0 3 0 0 6)

摘　　要：采用多步溶胀与悬浮聚合联用的方法 ,以 2 乙烯基吡啶 (2 VP)为功能单体 ,二乙二醇二丙烯酸酯(DEGDA)为交联剂制备出N 苯甲氧羰基 L 色氨酸 (N Cbz L Trp)为模板的单分散分子印迹聚合物。高效液相色谱表征显示 ,制备的单分散分子印迹聚合物在很短的色谱柱中就能够实现对模板分子对映异构体的基线分离。以乙腈为流动相 ,进样量 1 0 0 0ng ,流速 0 .2 5mL/min ,柱温 30℃时 ,该印迹聚合物对N 苯甲氧羰基 LDUniform-sized molecularly imprinted polymer for N-carbobenzoxy-L-tryptophan (N-Cbz-L-Trp) was prepared by a multi-step swelling and suspension polymerization method used 2-vinyl pyridine (2-VP) as functional monomer and diethylene glycol diacrylate (DEGDA) as cross-linker. The polymer was gel like particles whose average diameter is 6.3 mum and diameter distribution is 1.03. It was found that the baseline chiral separation of the template enantiomer was achieved on a shorter column. The separating degree was better, when the chromatographic conditions were as follows: acetonitrile as the mobile phase, sample amount 1000 ng, flow rate 0.25 mL/min and column temperature 30degreesC respectively.

关 键 词：N-苯甲氧羰基-L-色氨酸 单分散 分子印迹聚合物 手性拆分 高效液相色谱 固定相 

分 类 号：O657.7[理学—分析化学]