痕量还原性辅酶I的抑制荧光法测定  

Highly Sensitive Determination of Nicotinamide Adenine Dinueleotide by Inhibited Spectrofluorimetry

作　　者：陈亚红[1] 蔡汝秀[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析化学》2004年第6期719-723,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目 (No .2 0 2 75 0 2 7)

摘　　要：基于烟酰胺腺嘌呤二核苷酸 (简称辅酶I ,NADH)对血红蛋白催化H2 O2 氧化L 酪氨酸的反应具有强烈的抑制作用 ,提出了一种新的测定NADH的荧光分析法。该方法灵敏 ,简单 ,其线性范围为 5 .0× 1 0 -8～2 .0× 1 0 -6mol/L ;检出限为 2 .0× 1 0 -8mol/L。进一步探讨了NADH对血红蛋白催化反应的抑制机理 。A highly sensitive and simple spectrofluorimetric method for the determination of nicotinamide adenine dinucleotide (NADH) based on its inhibitory effect on hemoglobin catalytic reaction was developed. Under the optimum conditions, the degree of inhibitory effect was linear to the NADH concentration in the range of 5.0×10 -8 ～2.0×10 -6 mol/L. The relative standard deviation was 3.6% for eleven determinations of 2 0×10 -7 mol/L NADH and the detection limit was 2.0×10 -8 mol/L. Further experimental results revealed that the inhibition of NADH on this system was of the competitive type.

关 键 词：还原性辅酶I 痕量测定 抑制荧光法 血红蛋白 烟酰胺腺嘌呤二核苷酸 

分 类 号：O657.3[理学—分析化学]