脱氧核糖核酸在电极表面的固定化研究进展  

Advances of the Deoxyribonucleic Acid Immobilization on Electrode Surface for the Electrochemical Deoxyribonucleic Acid Biosensor Designing

作　　者：蔡宏[1] 徐颖[1] 何品刚[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《分析化学》2004年第6期815-820,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金 (No .2 9875 0 0 8)资助项目

摘　　要：介绍了电化学脱氧核糖核酸 (DNA)传感器的原理并重点评述了DNA在不同材料电极表面的固定化技术 ,对比了吸附法、自组装法、亲和素 生物素法、共价键合法以及组合法固定DNA在构建电化学DNA传感器中的应用。参考文献The immobilization of oligonucleotides on support has substantial applications in numerous cases, such as in the chemistry of nucleic acid, fabricating an electrochemical deoxyribonucleic acid ( DNA) biosensor and making the gene chip, etc.. The present report reviews immobilization techniques of DNA on the electrochemical transducer-electrode surface. The electrode is made from different substances such as carbon, gold, and platinumn, the immobilization techniques are ranged with direct adsorption, covalent bonding, self-assembling and avidin-biotin system. 77 references published after 1990 were cited.

关 键 词：脱氧核糖核酸 电极 固定化 电化学传感器 设计 

分 类 号：O657.1[理学—分析化学]