荣昌猪TYP基因克隆及其序列分析  

CLONING AND SEQUENCE ANALYSIS OF TYROSINASE GENE IN RONGCHANG PIG

作　　者：王金勇[1] 白小青[2] 范首君[1] 陈四清[1] 

机构地区：[1]重庆市养猪科学研究院,重庆荣昌402460 [2]西北农林科技大学,陕西杨凌712100

出　　处：《西南农业大学学报（自然科学版）》2004年第3期245-248,共4页Journal of Southwest Agricultural University

基　　金：国家十五攻关项目(2002BA514A-7-5);重庆市中青年科技专家基金资助项目(6852)

摘　　要：实验根据人、牛的酪氨酸酶(TYR,tyrosinase)基因序列设计了3对引物,扩增出猪的酪氨酸酶基因外显子2,3,4序列,其长度分别为210,135,182bp。对其进行了克隆测序,序列被GenBank收录,登录号分别为:AY236997,AY279998,AY242973。与人、牛、鼠的酪氨酸酶基因序列比对结果显示,TYR基因该区段在人、牛、鼠、猪上极其保守。Three pairs of primers were designed according to known DNA sequences tyrosinase in human and cattle, and exons 2, 3 and 4 were amplified of DNA sequences of tyrosinase in pig, which were 210, 135 and 182 bp in length. The PCR products were cloned and sequenced. Three sequences were authorized by GenBank with accession codes of AY 236997, AY 279998 and AY 242973, respectively. A comparison of the sequences with known DNA sequences in human, cattle and mice showed that the region of tyrosinase gene is highly conservative in human, cattle, mice and pigs.

关 键 词：酪氨酸酶基因 猪 毛色 

分 类 号：Q789[生物学—分子生物学] S828[农业科学—畜牧学]