应用实时定量PCR技术研究人重组干扰素α2b在人群中对麻疹病毒和风疹病毒的抑制作用  

Studies of the Effect of Interferon α2b on Inhibition of Measles Virus and Rubella Virus Replication by Using Real-time PCR Method

作　　者：舒跃龙[1] 周蕊[1] 刘丽琦[1] 史智扬[2] 朱凤才[2] 赵静[1] 李刚 唐震[2] 林里卓[1] 张丽兰 侯云德[1] 

机构地区：[1]病毒生物技术国家工程研究中心 [2]江苏省疾病预防控制中心,江苏南京210009 [3]徐州市铜山县疾病预防控制中心,江苏徐州221006 [4]北京远策药业有限责任公司,北京100176

出　　处：《病毒学报》2004年第2期166-168,共3页Chinese Journal of Virology

摘　　要：Human recombinant interferon α2b can inhibit replication of many viruses and it is an important prophylactic drug for virus infection. In this study, interferon α2b was used to study the biological function on measles virus and rubella virus replication in human. For each virus, selected population was divided into two groups, including with and without interferon α2b treatment, then the attenuated viral vaccine was used to inoculate each group and the respiratory samples were collected at fixed intervals. A highly sensitive and accurate Real-time PCR method was used to detect the virus copy number in samples. The results showed that the virus copy number was much lower after interferon treatment and suggested that interferon α2b could inhibit the measles virus and rubella virus replication in human.Human recombinant interferon α2b can inhibit replication of many viruses and it is an important prophylactic drug for virus infection. In this study, interferon α2b was used to study the biological function on measles virus and rubella virus replication in human. For each virus, selected population was divided into two groups, including with and without interferon α2b treatment, then the attenuated viral vaccine was used to inoculate each group and the respiratory samples were collected at fixed intervals. A highly sensitive and accurate Real-time PCR method was used to detect the virus copy number in samples. The results showed that the virus copy number was much lower after interferon treatment and suggested that interferon α2b could inhibit the measles virus and rubella virus replication in human.

关 键 词：实时定量PCR 干扰素 人群 麻疹 副粘病毒科 麻疹病毒 

分 类 号：R978.7[医药卫生—药品] Q78[医药卫生—药学]