大孔吸附树脂分离纯化人参二醇类和三醇类皂甙  

STUDY ON SEPARATION AND PURIFICATION PROCESS OF PANAXDIOL TYPE GINSENOSIDES AND PANAXTRIOL TYPE GINSENOSIDES WITH MACROPOROUS ADSORPTION RESIN

作　　者：赵瑜[1] 陈波[1] 罗旭彪[1] 张斐[1] 姚守拙[1] 

机构地区：[1]湖南师范大学化学化工学院,长沙410081

出　　处：《天然产物研究与开发》2004年第3期235-238,共4页Natural Product Research and Development

基　　金：国家"十五"攻关项目资助 ( 2 0 0 1BA746C ;2 0 0 1BA80 4A2 1)

摘　　要：本实验研究大孔吸附树脂从人参根提取物中富集、分离人参二醇类和人参三醇类皂甙的工艺条件及参数。用不同浓度的乙醇洗脱 ,使人参二醇类和三醇类皂甙实现富集分离 ,人参二醇类皂甙富集在 80 %乙醇洗脱液部分 ,人参三醇类皂甙富集在 4 0 %洗脱液部分。得到含量大于 2 5 %的人参三醇类皂甙 ,含量大于5 0 %的人参二醇类皂甙 ,总皂甙洗脱率在 91%以上。此法能够较好地分离、纯化人参二醇类和三醇类皂甙。The technical parameters for purification of panaxdiol type ginsenosides and panaxtriol type ginsenosides with macroporous adsorption resin were studied.Panaxtriol type ginsenosides and panaxdiol type ginsenosides were absorbed on macroporous adsorption resin,and eluted by different concentrations of ethanol.Panaxdiol type ginsenosides were enriched in the 80% ethanol,panaxtriol type ginsenosides in the 40% ethanol.With this method,the content of panaxdiol type ginsenosides and panaxtriol type ginsenosides can reach higher than 50% and 25% respectively,and the total recovery is higher than 91%.This method would be applied to separate mixture of panaxdiol type ginsenosides and panaxtriol type ginsenosides.

关 键 词：大孔吸附树脂 分离 纯化 人参二醇类皂甙 人参三醇类皂甙 

分 类 号：R284[医药卫生—中药学] Q949.763.2[医药卫生—中医学]