中华绒螯蟹大颚器分泌物的HPLC分析  

High Performance Lipid Chromatography(HPLC) Analysis of Secretion by Mandibular organ in Chinese Mitten-handed Crab (Eriocheir sinensis)

作　　者：杜育哲[1] 顾景龄[1] 吕宪禹[2] 王良臣[2] 

机构地区：[1]天津农学院水产科学系,天津300384 [2]天津南开大学生命科学院,天津300071

出　　处：《水产科学》2004年第6期10-13,共4页Fisheries Science

基　　金：天津农学院青年基金资助项目

摘　　要：利用三相提取法和高效液相色谱(HPLC)对中华绒螯蟹血淋巴及大颚器(MO)提取物进行了定性、定量的分析。结果发现在血淋巴及MO提取物中基本都可检测到三种物质:保幼激素Ⅲ(JHⅢ)、A和B。A物质的分泌受到眼柄因子的抑制调控,可能就是中华绒螯蟹MO的分泌物—法呢酸甲酯(MF)。Mandibular organ and its secretion were analysed qualitatively and quantitatively in hemolymph in Eriocheir sinensis using triphasic extraction procedure with normal phase high performance lipid chromatography (HPLC). Three products were detected: JH III、A and B. The level of A, which may be methyl farnesoate (MF), in hemolyph increased after eyestalk ablation.

关 键 词：中华绒螯蟹 大颚器 HPLC 分泌物 

分 类 号：S968[农业科学—水产养殖]