围植入期小鼠子宫内膜中整合素β_3亚基的表达  

EXPRESSION OF INTEGRIN β_3 SUBUNIT IN PERI-IMPLANTATION MOUSE ENDOMETRIUM

作　　者：卢桂兰[1] 武玉玲[2] 邴鲁军[2] 宋卫华[2] 朱传菊[2] 

机构地区：[1]北京市疾病预防控制中心传染病与地方病控制所,北京100013 [2]山东大学医学院组织胚胎学教研室,济南250012

出　　处：《中国组织化学与细胞化学杂志》2004年第2期164-167,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：山东省自然科学基金资助课题 (Y2 0 0 1C33)

摘　　要：目的　研究整合素β3 亚基与胚泡植入的相关关系。方法　取未孕动情期、真孕及假孕 3- 6天小鼠子宫作切片 ,用免疫组织化学和图像分析法检测整合素 β3 亚基围植入期子宫内膜的表达状况和变化规律。结果　整合素 β3 亚基仅分布于子宫内膜腺上皮的细胞膜。在真孕组小鼠 ,受精后第 4天 ,整合素β3 亚基含量明显上升 ,第 5天达到高峰 ,第 6天又突然回落 ;假孕组小鼠 ,只有第 5天时呈弱阳性表达 ,其余各天的表达均降至检测水平之下。结论　①整合素 β3 亚基在真孕小鼠子宫内膜的表达与植入窗的开放相吻合 ,是子宫内膜接受性的客观标志。②启动整合素 β3 亚基表达变化的信号不仅受母体因素调控 ,还与胚泡刺激有关。Objective To study the relationship between integrin β 3 subunit and blastocyst implantation. Method The uteri of nonpregnant (estrous stage) and 3～6d pregnant and pseudopregnant mice were sectioned. The distribution and changes of integrin β 3 subunit in the peri-implantation mouse endometrium were examined by immunohistochemistry. An image analysis was also performed. Result The integrin β 3 subunit-specific staining was restricted to the membrane of endometrial glandular epithelial cells only. In the pregnant group, the level of integrinβ 3 increasedconsiderably on the4th day of gestation, peaked on day 5, and then decreased obviously on day 6. In the pseudopregnant group, weak staining of integrin β 3 was only observed on the 5th dayof gestation, while integrinβ 3 level fell to below detection limits on the other days. Conclusion ①The expression of integrin β 3 subunit in pregnant mouse endometrium frames the'implantation window', which is a candidate biochemical marker of uterine receptivity. ②The signal inducing the expression of integrin β 3 subunit in mouse endometrium is regulated not only by maternal factors, but also by the implanted embryos.

关 键 词：胚泡 围植入期 整合素Β3亚基 子宫内膜 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]