沼泽红假单胞菌偶氮还原酶新基因的克隆表达  

Plasmid Construction and Expression of A New Azoreductase Gene from Rhodopseudomonas palustris Gene in E.coli

作　　者：李可[1] 张肇铭[1] 刘晓辉[1] 熊琦[1] 裴雁曦[1] 

机构地区：[1]山西大学生命科学与技术学院,山西太原030006

出　　处：《生物技术》2004年第3期8-10,共3页Biotechnology

基　　金：国家科技攻关项目 (2 0 0 1BA540C"光合细菌产品开发")

摘　　要：前期实验证明沼泽红假单胞菌 (Rhodopsedomonaspalustris)对偶氮染料有较强的降解能力 ,通过PCR扩增 ,从该菌质粒DNA中扩增获得了一条未登录新基因PAR - 1。将该基因构建到融合蛋白表达载体pGEX - 4T - 1,通过IPTG诱导 ,进行原核蛋白表达 ,SDS -PAGE电泳显示 ,有约 4 8kD融合蛋白表达。对经诱导的大肠杆菌 (BL2 1)进行偶氮染料脱色试验 。In previous work,it was demonstrated that Rhodopsedomonas palustris could well degrade azo dyes.a novel gene,named PAR-1 was obtained by PCR from the plasmid of this strain.In this paper,this gene was inserted into exprssing vector pGEX-4T-1,which could express a GST fusion protein in E.coli strain BL21(DE3) by IPTG inducing.The target fusion protein about 48kD was detected by SDS-PAGE.Slingt azoreductase activity was obsearved in E.coli cell suspension.

关 键 词：沼泽红假单胞菌 偶氮还原酶 融合表达 

分 类 号：Q786[生物学—分子生物学]