悬铃木体细胞胚胎发生及植株再生  

Somatic Embryogenesis of Platanus orientalis and Its Plantlet Regeneration

作　　者：范国强[1] 黎明[1] 贺窑青[1] 蒋建平[1] 

机构地区：[1]河南农业大学,郑州450002

出　　处：《林业科学》2004年第3期71-74,i001,共5页Scientia Silvae Sinicae

基　　金：河南省杰出人才创新基金 ( 3 2 10 0 170 0 );高校杰出科研人才工程资助项目 ( 2 0 0 2KYCX0 0 3 )的部分内容

摘　　要：以叶片为材料 ,研究了悬铃木的体细胞胚胎发生及其植株再生。结果表明 ,悬铃木幼苗叶片在WPM +0 1mg·L- 1 IBA +5 0mg·L- 1 BA培养基上的愈伤组织诱导率和胚性愈伤组织诱导率分别为 10 0 %和 89 1% ;2 5℃的温度有利于胚性愈伤组织在WPM +0 5mg·L- 1 BA培养基上进行体细胞胚胎发生 ;黑暗低温处理 (10℃ ) 3d不但可以促进体细胞胚胎的发生过程 ,而且可以提高其发生频率。The somatic embryogenesis and plantlet regeneration from the leaf callus of Platanus orientalis were studied in this paper. The results indicated that the callus and embryogenic callus(EC) frequency on the WPM0.1 mg5L -1 IBA5.0 mg5L -1 BA medium were 100% and 89.1% respectively. The procedure of somatic embryogenesis of EC on WPM0.5 mg5L -1 BA medium at 25℃ might be promoted. Not only could the stages of somatic embryogenesis on all media in the experiment be promoted, but also the frequency of the somatic embryogenesis might be enlarged.

关 键 词：悬铃木 体细胞胚胎发生 植株再生 愈伤组织 培养基 

分 类 号：S792.37[农业科学—林木遗传育种]