重组人γ干扰素复性过程中体外活性检测方法研究  

A Colorimetric Method to Assay Biological Activity of Recombinant Human IFN-γ

作　　者：费峥峥[1] 关怡新[1] 姚善泾[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,杭州310027

出　　处：《微生物学通报》2004年第3期65-69,共5页Microbiology China

基　　金：浙江省自然科学基金资助项目(No.201099);教育部留学回国人员科研启动基金资助项目

摘　　要：结晶紫染色法由于具有客观、精确的特点,适用于大量抗肿瘤药物的药敏测定。本实验对此方法进行了探讨和条件优化,考察了结晶紫染液的用量及作用时间、溶媒提取时间、孔板效应及边缘效应等,并将其应用于重组人γ干扰素包涵体初步复性的体外活性检测之中。The colorimetric method has been well developed to detect the sensitivity of most antiproliferative drugs with the advantages of objectivity and accuracy. Many parameters including the dosage and the absorbance of crystalline violet, the extraction time of the solvent, plate effect and marginal effect were investigated, then the optimized method was applied further to measure the biological activity during the refolding of recombinant human IFN-γ inclusion bodies.

关 键 词：结晶紫染色法 Γ干扰素 活性检测 复性 

分 类 号：R927[医药卫生—药学]