一个从顶头孢霉中筛选具有启动子功能的DNA片段的简便方法  

A Convenient Method to Select DNA Fragments of Cephalosporium acremonium with Promoter Function

作　　者：张丕燕[1] 朱春宝[1] 朱宝泉[1] 赵文杰[1] 

机构地区：[1]上海医药工业研究院,上海200040

出　　处：《微生物学通报》2004年第3期97-100,共4页Microbiology China

基　　金：国家技术研究发展计划(No.2001AA214201)

摘　　要：利用大肠杆菌-酵母穿梭质粒pGBT9构建了一个真菌启动子捕获载体pGBT14,用这个捕获载体构建了含顶头孢霉的0.5-2.0kb片段的染色体DNA文库,并从中筛选到顶头孢霉的24个DNA片段,这些片段能够在啤酒酵母中启动Trp1基因的表达。A promoter-trap vector pGBT14 for selecting promoters of fungus gene was constructed with E. coli-yeast shuttling plasmid pGBT9. Using this vector, a0. 5-2. 0kb chromosomal DNA library of Cepholosporium acremonium was constructed, and twenty four DNA fragments with promoter function in Saccharomyces oerevisiae Y153 were selected from this DNA library. And the promoter function of these DNA fragments was analyzed.

关 键 词：大肠杆菌-酵母穿梭质粒 启动子捕获载体 顶头孢霉 DNA文库 真菌 

分 类 号：Q93[生物学—微生物学]