南昌霉素A产生菌原生质体诱变选育研究  

Strain Improvement of Nanchangmycin A-Production by Protoplast Mutagenesis

作　　者：魏赛金[1] 程新[1] 涂国全[1] 

机构地区：[1]江西农业大学生物工程系,江西南昌330045

出　　处：《江西农业大学学报》2004年第3期427-430,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：山东胜利股份有限公司资助项目

摘　　要：南昌霉素A(NanchangmycinA)产生菌——南昌链霉菌(Streptomycesnanchangensis)的原生质体经不同浓度NTG处理以后,分别采用抗药性诱变筛选和随机筛选选育高产菌株,通过连续3批次摇瓶发酵筛选出高产菌株400-N-4s,其产南昌霉素A的能力比出发菌株116-3r-68提高了20%以上。同时,经过对两种筛选结果进行统计学分析,结果表明,采用抗药性致死标记技术,可以有效的提高抗生素高产菌株的选育效率,得到较好的结果。Protoplast of the Streptomyces nanchangensis strain was mutated by different dosage of NTG and then selected with resistance mutational screening and random screening respectively,a high-yeilding strain 400-N-4s was obtained. Through the continuous 3 batches fermentation,its Nanchangmycin -A production is 20% higher than that of the parent strain 116-3r-68.The results of statistical analysis indicated that the the resistance mutational screening is a better approach to increase the yield of antibiotics.

关 键 词：南昌霉素A 南昌链霉菌 原生质体 诱变 

分 类 号：Q813.5[生物学—生物工程]