共聚焦激光扫描显微镜活体观察动脉粥样硬化家兔模型大脑皮质血管内血液缘流厚度的改变  

Observe the Changing of Edge-flowing Blood Protective Barrier under the Confocal Laser Scanning Microscopy within the Cerebral Cortex Arteries of Rabbit with Atherosclerosis in Vivo

作　　者：乡志雄[1] 谢泽宇[1] 庄明华[1] 唐永红[2] 邢达[2] 

机构地区：[1]汕头大学医学院第二附属医院神经外科,中国广东汕头515041 [2]华南师范大学激光生命科学研究所,中国广东广州510631

出　　处：《激光生物学报》2004年第3期190-193,共4页Acta Laser Biology Sinica

基　　金：广东省科技计划项目课题(200044)

摘　　要：目的:探讨在兔脑皮层动脉粥样硬化时血管内缘流厚度的变化。方法:用共聚焦激光扫描显微镜比较家兔脑皮质动脉内缘流厚度在正常、粥样硬化病理状态下的不同。结果:在血管直径为74.87±3.26μm的血管中血液缘流厚度在病理状态下随血管的舒张、收缩而发生改变;而在血管直径为94.33±2.84μm的缘流厚度在血管收缩时发生改变。结论:动脉粥样硬化的家兔模型中血液缘流厚度随血液的流变性质改变而改变。Objective: To observed the morphological and thickness changing of the protective barrier formed by the edge-flowing blood in vessels within the cerebral cortex arteries with atherosclerosis. Method: We have used confocal laser scanning microscopy, dynamically measure the thickness of the protective barrier formed by the cedge-flowing blood in vessels within the cerebral cortical arteries of rabbits in the normal contrast group and atherosclerotic group under sitmulation with Ligustrazine(or Tetramethylpyrazine) or Noradrenalin. Result: In the vessel which diameter is 74.87±3.26μm, the thickness of the protective barrier formed by the edge-flowing blood in vessels was difference between the pathological group and the control group. And the thickness of the edge-flowing blood layerchanged between the vessels were constricting or diastolic in the vessel that diameter was 94.33±(2.84μm.) Conclusion:The thickness of protective barrier formed by the dge-flowing blood in vessels is closely related to atherosclerosis.

关 键 词：激光共聚焦扫描显微镜 血液动力学 动脉粥样硬化 血液缘流保护性屏障 

分 类 号：Q631[生物学—生物物理学] R318.51[医药卫生—生物医学工程]